Harkness Linda, Christiansen Helle, Nehlin Jan, Barington Torben, Andersen Jens S, Kassem Moustapha
Department of Endocrinology and Metabolism, Medical Biotechnology Center, University of Southern Denmark, Odense C, Denmark.
Stem Cell Res. 2008 Sep;1(3):219-27. doi: 10.1016/j.scr.2008.06.001. Epub 2008 Jul 8.
Proteomic profiling of human embryonic stem cells (hESC) can identify cell fate determination and self-renewal biomarkers. Employing Fourier transform LC-ESI-MS/MS and MS(3) mass spectrometry, we obtained a membrane proteomic signature overlapping between hESC cultured on mouse embryonic fibroblast (MEF) feeders and those grown under MEF-free culture conditions. We identified 444 transmembrane or membrane-associated proteins, of which 157 were common between both culture conditions. Functional annotation revealed CD antigens (10%), adhesion proteins (4%), proliferation-associated proteins (4%), receptors (41%), transport proteins (21%), structural proteins (5%), and proteins with miscellaneous functions (15%). In addition, 15 CD antigens and a number of surface marker molecules not previously observed in hESC at a proteome level, e.g., Nodal modulator 1, CD222, transgelin-2, and CD81, were identified. In conclusion, we describe the first membrane proteome profile of hESC that is independent of culture conditions. These data can be used to define the phenotype of hESC.
对人类胚胎干细胞（hESC）进行蛋白质组分析能够识别细胞命运决定和自我更新生物标志物。利用傅里叶变换液相色谱-电喷雾串联质谱法（LC-ESI-MS/MS）和三级质谱法（MS(3)），我们获得了在小鼠胚胎成纤维细胞（MEF）饲养层上培养的hESC与在无MEF培养条件下生长的hESC之间重叠的膜蛋白质组特征。我们鉴定出444种跨膜或膜相关蛋白，其中157种在两种培养条件下都存在。功能注释显示包括CD抗原（10%）、黏附蛋白（4%）、增殖相关蛋白（4%）、受体（41%）、转运蛋白（21%）、结构蛋白（5%）以及具有其他功能的蛋白（15%）。此外，还鉴定出15种CD抗原以及一些之前在hESC蛋白质组水平未观察到的表面标志物分子，例如Nodal调节因子1、CD222、原肌球蛋白-2和CD81。总之，我们描述了首个与培养条件无关的hESC膜蛋白质组图谱。这些数据可用于定义hESC的表型。
