Department of Biochemistry, Medical College of Wisconsin, Milwaukee, WI 53226, USA.
Proteomics. 2011 Oct;11(20):3947-61. doi: 10.1002/pmic.201100100. Epub 2011 Sep 8.
Stem cells represent obvious choices for regenerative medicine and are invaluable for studies of human development and drug testing. The proteomic landscape of pluripotent stem cells (PSCs), in particular, is not yet clearly defined; consequently, this field of research would greatly benefit from concerted efforts designed to better characterize these cells. In this concise review, we provide an overview of stem cell potency, highlight the types and practical implications of heterogeneity in PSCs and provide a detailed analysis of the current view of the pluripotent proteome in a unique resource for this rapidly evolving field. Our goal in this review is to provide specific insights into the current status of the known proteome of both mouse and human PSCs. This has been accomplished by integrating published data into a unified PSC proteome to facilitate the identification of proteins, which may be informative for the stem cell state as well as to reveal areas where our current view is limited. These analyses provide insight into the challenges faced in the proteomic analysis of PSCs and reveal one area--the cell surface subproteome--that would especially benefit from enhanced research efforts.
干细胞是再生医学的明显选择，对于人类发育和药物测试的研究具有不可估量的价值。特别是多能干细胞 (PSCs) 的蛋白质组景观尚未明确界定；因此，这一研究领域将极大地受益于协调一致的努力，旨在更好地描述这些细胞。在这篇简明的综述中，我们概述了干细胞的多能性，强调了 PSCs 异质性的类型和实际意义，并对这一快速发展领域的多能蛋白质组的当前观点进行了详细分析。我们在这篇综述中的目标是为人们提供关于已知的小鼠和人类 PSCs 蛋白质组的现状的具体见解。这是通过将已发表的数据整合到一个统一的 PSC 蛋白质组中实现的，以便于鉴定可能对干细胞状态具有信息性的蛋白质，并揭示我们当前观点有限的领域。这些分析深入了解了 PSCs 蛋白质组分析所面临的挑战，并揭示了一个特别需要加强研究的领域——细胞表面亚蛋白质组。
