三次谐波产生显微镜术

Third harmonic generation microscopy.

作者信息

Squier J, Muller M, Brakenhoff G, Wilson K R

出版信息

Opt Express. 1998 Oct 26;3(9):315-24. doi: 10.1364/oe.3.000315.

DOI:10.1364/oe.3.000315

PMID:19384376

Abstract

Third harmonic generation microscopy is used to make dynamical images of living systems for the first time. A 100 fs excitation pulse at 1.2 aem results in a 400 nm signal which is generated directly within the specimen. Chara plant rhizoids have been imaged, showing dynamic plant activity, and non-fading image characteristics even with continuous viewing, indicating prolonged viability under these THG-imaging conditions.

摘要

首次使用三次谐波产生显微镜对生物系统进行动态成像。1.2 埃米处的100飞秒激发脉冲产生了直接在样本内生成的400纳米信号。已经对轮藻属植物的假根进行了成像，显示出动态的植物活动，并且即使持续观察也具有不褪色的图像特征，表明在这些三次谐波产生成像条件下具有延长的活力。