del Olmo Nuria, Gramage Esther, Alguacil Luis F, Pérez-Pinera Pablo, Deuel Thomas F, Herradón Gonzalo
Laboratory of Pharmacology and Toxicology, Universidad San Pablo CEU, Madrid, Spain.
Growth Factors. 2009 Jun;27(3):189-94. doi: 10.1080/08977190902906859.
Pleiotrophin (PTN) is a growth factor that has been shown to be involved in hippocampal synaptic plasticity and learning. To further understand the involvement of PTN in memory processes, we performed in vitro electrophysiological studies in PTN-stimulated CA1 from rat hippocampal slices combined with the behavioural testing of PTN deficient (PTN - / - ) mice. We found that PTN inhibited hippocampal long-term potentiation (LTP) induced by high-frequency stimulation (HFS) consisted in three trains of 100 Hz separated by 20 s. To test the possibility that PTN might be involved in behavioural memory processes, we tested the learning behaviour of PTN - / - mice using the Y-maze test. We did not observe significant differences in recognition memory between PTN - / - and Wild Type (WT) mice when a 30 min-interval intertrial (ITI) was used in the Y-maze test. However, whereas WT mice showed disruption of recognition memory using a 60 min-ITI, PTN - / - mice maintained the recognition memory. The data demonstrate that PTN inhibits hippocampal LTP in vitro and might play a role in memory processes in vivo.
多效生长因子（PTN）是一种生长因子，已被证明与海马体突触可塑性和学习有关。为了进一步了解PTN在记忆过程中的作用，我们对大鼠海马切片中经PTN刺激的CA1区进行了体外电生理研究，并对PTN基因敲除（PTN - / - ）小鼠进行了行为测试。我们发现，PTN抑制了由高频刺激（HFS）诱导的海马体长期增强效应（LTP），高频刺激由三串100 Hz的刺激组成，间隔20秒。为了测试PTN可能参与行为记忆过程的可能性，我们使用Y迷宫测试对PTN - / - 小鼠的学习行为进行了测试。当在Y迷宫测试中使用30分钟的试验间隔（ITI）时，我们没有观察到PTN - / - 小鼠和野生型（WT）小鼠在识别记忆方面有显著差异。然而，当使用60分钟的ITI时，WT小鼠的识别记忆受到破坏，而PTN - / - 小鼠则保持了识别记忆。数据表明PTN在体外抑制海马体LTP，并且可能在体内记忆过程中发挥作用。
