Hubei Key Laboratory of Natural Medicinal Chemistry and Resource Evaluation, College of Pharmacy, Huazhong University of Science and Technology, Hangkong Road 13, Wuhan 430030, China.
Hubei Key Laboratory of Natural Medicinal Chemistry and Resource Evaluation, College of Pharmacy, Huazhong University of Science and Technology, Hangkong Road 13, Wuhan 430030, China.
Carbohydr Polym. 2017 Oct 1;173:215-222. doi: 10.1016/j.carbpol.2017.05.076. Epub 2017 May 27.
Structures and in vitro hypoglycemic activities of polysaccharides from different species of Maidong were studied. The primary structures of polysaccharides were elucidated on the basis of GC, GC-MS, infrared, NMR and periodate oxidation-Smith degradation. Liriope spicata polysaccharide (LSP), Ophiopogon japonicus polysaccharide (OJP) and Liriope muscari polysaccharide (LMP) were composed of β-fructose and α-glucose. The average molecular weights of LSP, OJP and LMP were 4742, 4925 and 4138Da with polydispersity indexes of 1.1, 1.2 and 1.1, respectively. The backbones of polysaccharides were formed by Fruf-(2→, →2)-Fruf-(6→, →6)-Glcp-(1→ and →1, 2)-Fruf-(6→ with a molar ratio of 5.0:18.2:1.0:5.3 (LSP), 6.8:15.8:1.0:5.8 (OJP), 8.3:12.3:1.0:3.9 (LMP), respectively. The RT-PCR and western blot analysis indicated that LSP, LMP and OJP increased the expression of PI3K, AKT, InsR, PPARγ and decreased the expression of PTP1B in mRNA level and protein level in IR HepG2 cells. Furthermore, glucose consumption was increased after treated with polysaccharides. These results revealed that LSP, OJP and LMP had potential anti-diabetic effects.
研究了不同种麦冬多糖的结构和体外降血糖活性。根据 GC、GC-MS、红外、NMR 和过碘酸-Smith 降解，阐明了多糖的一级结构。石刁柏多糖（LSP）、麦冬多糖（OJP）和山麦冬多糖（LMP）由β-果糖和α-葡萄糖组成。LSP、OJP 和 LMP 的平均分子量分别为 4742、4925 和 4138Da，多分散指数分别为 1.1、1.2 和 1.1。多糖的骨架由 Fruf-(2→, →2)-Fruf-(6→, →6)-Glcp-(1→和→1, 2)-Fruf-(6→组成，摩尔比分别为 5.0:18.2:1.0:5.3（LSP）、6.8:15.8:1.0:5.8（OJP）、8.3:12.3:1.0:3.9（LMP）。RT-PCR 和 Western blot 分析表明，LSP、LMP 和 OJP 增加了 IR HepG2 细胞中 PI3K、AKT、InsR、PPARγ 的表达，并降低了 PTP1B 的表达。此外，经多糖处理后葡萄糖消耗增加。这些结果表明 LSP、OJP 和 LMP 具有潜在的抗糖尿病作用。
