Kumada Y, Imai S, Nagaoka K
Pharmaceutical Research Center, Meiji Seika Kaisha, Ltd., Yokohama, Japan.
J Antibiot (Tokyo). 1991 Sep;44(9):1006-12. doi: 10.7164/antibiotics.44.1006.
Two oligopeptides containing phosphinothricin were accumulated in the culture of bialaphos (BA) producer Streptomyces hygroscopicus SF1293 when large amount of BA was added to the culture at idio phase. One of the oligopeptides was a new substance, BA dimer (phosphinothricyl-alanyl-alanyl-phosphinothricyl-alanyl-alan ine), and the other was a known substance called If (phosphinothricyl-alanyl-alanyl-phosphinothricin). Though BA none producing mutants which were blocked at the steps 1, 10 and 13, respectively in BA biosynthesis also converted BA to BA dimer and If, the mutants blocked at the step 11 (alanylation) could not curry out the conversion. Antibiotic activities of BA dimer and If were lower than 1/100 of BA.
在抗除草剂双丙氨膦（BA）产生菌吸水链霉菌SF1293的培养中，当在生长后期向培养物中添加大量BA时，积累了两种含草丁膦的寡肽。其中一种寡肽是新物质BA二聚体（草丁膦基 - 丙氨酰 - 丙氨酰 - 草丁膦基 - 丙氨酰 - 丙氨酸），另一种是已知物质If（草丁膦基 - 丙氨酰 - 丙氨酰 - 草丁膦）。虽然在BA生物合成中分别在步骤1、10和13受阻的不产生BA的突变体也能将BA转化为BA二聚体和If，但在步骤11（丙氨酰化）受阻的突变体不能进行这种转化。BA二聚体和If的抗生素活性低于BA的1/100。
