[枯草芽孢杆菌SBS液体培养联产溶栓酶和γ-聚谷氨酸]
[Co-production of thrombolytic enzyme and gamma-polyglutamic acid by liquid-culture of Bacillus subtilis SBS].
作者信息
Hu Chungang, Liu Changjin, Zheng Huanlan, Zhou Ping
机构信息
TEDA BIO-X Center for Systems Biotechnology, College of Food Engineering and Biotechnology, Tianjin University of Science Technology, Tianjin 300457, China.
出版信息
Wei Sheng Wu Xue Bao. 2009 Jan;49(1):49-55.
OBJECTIVE
To analyze the co-production of thrombolytic enzyme and gamma-polyglutamic acid by liquid-culture of Bacillus subtilis SBS.
METHODS
We used Bacillus subtilis SBS to produce thrombolytic enzyme (BTE) and 7-polyglutamic acid (gamma-PGA) by liquid fermentation. We conducted orthogonal experiments analysis between carbon source and nitrogen source. Then through Fibrin-SDS-PAGE, UV spectrum, Infra-Red spectrum and high performance liquid chromatography (HPLC), we identified the production.
RESULTS
We synthesized gamma-PGA in the medium without the addition of L-glutamic acid. Bacillus subtilis SBS was a L-glutamic acid-independent bacterium. The suitable carbon and nitrogen sources for the synthesis of thrombolytic enzyme (BTE) were soluble starch and soybean peptone. However,the suitable carbon and nitrogen sources for the synthesis of gamma-PGA were sucrose and NH4Cl.
CONCLUSION
The yields of both products could close to the levels of the single synthesis when the concentrations of sucrose, soybean peptone and NH4Cl were 10, 20, 8 g/L respectively, the activity of BTE reached 265 +/- 25 IU/mL,and the concentration of gamma-PGA reached 1.183 +/- 0.015 g/L.
目的
通过枯草芽孢杆菌SBS的液体培养分析溶栓酶和γ-聚谷氨酸的联产情况。
方法
我们利用枯草芽孢杆菌SBS通过液体发酵生产溶栓酶（BTE）和γ-聚谷氨酸（γ-PGA）。我们对碳源和氮源进行了正交实验分析。然后通过纤维蛋白-SDS-PAGE、紫外光谱、红外光谱和高效液相色谱（HPLC）对产物进行鉴定。
结果
在不添加L-谷氨酸的培养基中合成了γ-PGA。枯草芽孢杆菌SBS是一种不依赖L-谷氨酸的细菌。合成溶栓酶（BTE）的适宜碳源和氮源是可溶性淀粉和大豆蛋白胨。然而，合成γ-PGA的适宜碳源和氮源是蔗糖和氯化铵。
结论
当蔗糖、大豆蛋白胨和氯化铵的浓度分别为10、20、8 g/L时，两种产物的产量可接近单产水平，BTE的活性达到265±25 IU/mL，γ-PGA的浓度达到1.183±0.015 g/L。
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