State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi University, Nanning 530004, Guangxi, China.
Bioresour Technol. 2013 Aug;142:697-700. doi: 10.1016/j.biortech.2013.05.020. Epub 2013 May 16.
Poly(γ-glutamic acid), as a naturally occurring homopolymer, is widely used in industry, agriculture, food and medicine. Fibrinolytic enzyme has a great potential for the prevention and/or treatment of vascular diseases caused by fibrin clots. Co-production of γ-PGA and fibrinolytic enzyme by Bacillus subtilis GXA-28 (CCTCC M 2012347) from soybean residue using cane molasses and monosodium glutamate waste liquor under sterilized and non-sterilized condition were investigated. It was observed that total sugar from cane molasses of 3% (w/w) and glutamate from monosodium glutamate waste liquor of 2% (w/w) were favorable for γ-PGA and fibrinolytic enzyme co-production at pH 7.0 and 45°C. Based on the optimal medium, the γ-PGA and fibrinolytic activity reached 103.5 g/kg-substrates at 22 h and 986 U/g-substrates at 24h under non-sterilized condition, respectively. To our knowledge, the yield of γ-PGA was highest in all reported literatures.
聚谷氨酸是一种天然存在的均聚物，广泛应用于工业、农业、食品和医药领域。纤溶酶在预防和/或治疗由纤维蛋白凝块引起的血管疾病方面具有巨大的潜力。从大豆残渣中用甘蔗糖蜜和谷氨酸钠废液生产枯草芽孢杆菌 GXA-28（CCTCC M 2012347）的γ-PGA 和纤溶酶的共生产，在灭菌和非灭菌条件下进行了研究。结果表明，甘蔗糖蜜中的总糖为 3%（w/w），谷氨酸钠废液中的谷氨酸为 2%（w/w），在 pH7.0 和 45°C 时有利于γ-PGA 和纤溶酶的共生产。基于最佳培养基，在非灭菌条件下，22 小时达到 103.5 g/kg-基质的γ-PGA 和 24 小时达到 986 U/g-基质的纤溶酶活。据我们所知，γ-PGA 的产率在所有报道的文献中是最高的。
