Memmi A, Sansa G, Rjeibi I, El Ayeb M, Srairi-Abid N, Bellasfer Z, Fekhih A
Faculté de Pharmacie, rue Avicenne 5009, Monastir, Tunisie.
Arch Inst Pasteur Tunis. 2007;84(1-4):49-55.
The scorpionic and ophidian envenomations are a serious public health problem in Tunisia especially in Southeastern regions. In these regions Artemisia campestris L is a plant well known which has a very important place in traditional medicine for its effectiveness against alleged venom of scorpions and snakes. In this work, we tested for the first time, the anti-venomous activity of Artemisia campestris L against the scorpion Androctonus australis garzonii and the viper Macrovipera lebetina venoms. Assays were conducted by fixing the dose of extract to3 mg/mouse while doses of venom are variable. The leaves of Artemisia campestris L were extracted by various organic solvents (Ether of oil, ethyl acetate, methanol and ethanol) and each extract was tested for its venom neutralizing capacity. For the ethanolic extract, a significant activity with respect to the venoms of scorpion Androctonus australis garzonii (Aag), was detected. Similarly, a significant neutralizing activity against the venom of a viper Macrovipera lebetina (Ml), was obtained with the dichloromethane extract. These results suggest the presence of two different type of chemical components in this plant: those neutralizing the venom of scorpion are soluble in ethanol whereas those neutralizing the venom of viper are soluble in dichloromethane.
在突尼斯，尤其是东南部地区，蝎子和毒蛇咬伤是一个严重的公共卫生问题。在这些地区，原野蒿是一种知名植物，因其对所谓的蝎子和蛇毒具有疗效，在传统医学中占有非常重要的地位。在这项研究中，我们首次测试了原野蒿对澳氏肥尾蝎和草原蝰蛇毒液的抗毒活性。实验通过将提取物剂量固定为3毫克/小鼠，同时改变毒液剂量来进行。用各种有机溶剂（石油醚、乙酸乙酯、甲醇和乙醇）提取原野蒿的叶子，并对每种提取物的毒液中和能力进行测试。对于乙醇提取物，检测到其对澳氏肥尾蝎（Aag）毒液具有显著活性。同样，二氯甲烷提取物对草原蝰蛇（Ml）毒液具有显著的中和活性。这些结果表明该植物中存在两种不同类型的化学成分：中和蝎子毒液的成分可溶于乙醇，而中和蝰蛇毒液的成分可溶于二氯甲烷。
