Ozkan Ozcan, Carhan Ahmet
Refik Saydam Hygiene Center, Poison Research Center, 06100 Ankara, Turkey.
Toxicon. 2008 Aug 1;52(2):375-9. doi: 10.1016/j.toxicon.2008.06.019. Epub 2008 Jun 26.
Scorpion envenomations are considerable health problem in tropical and subtropical regions. There are approximately 1,500 species of scorpions worldwide. The number of dangerous species in the Buthidae family is significantly higher than in other families of scorpions. Mesobuthus eupeus is a member of Mesobuthus genus, Buthidae family. In this study, the potency and para-specific activities of Androctonus crassicauda antivenom were investigated against M. eupeus scorpion venom. The median lethal dose of M. eupeus and A. crassicauda scorpion venoms were found to be 0.18 mg/kg, 15.45 microg/kg by i.c.v injection route. The antivenom showed neutralization effect against the venoms of M. eupeus. One milliliter of A. crassicauda antivenom neutralizes 464LD(50) of M. eupeus and 940LD(50)A. crassicauda venom on mice. Western blotting demonstrated immunologic reaction with the venoms. The monovalent antivenom has immunoactivity and neutralizing capacity to the scorpion venoms. This study indicates that the antivenom produced by Refik Saydam Hygiene Center could be used for the treatment of M. eupeus stings in Turkey.
蝎子蜇伤在热带和亚热带地区是一个相当严重的健康问题。全世界大约有1500种蝎子。钳蝎科中的危险物种数量明显高于其他蝎子科。中东金蝎是钳蝎科中东金蝎属的成员。在本研究中，对粗尾蝎抗蛇毒血清针对中东金蝎蝎毒的效力和种间特异性活性进行了研究。通过脑室内注射途径，发现中东金蝎和粗尾蝎蝎毒的半数致死剂量分别为0.18mg/kg、15.45μg/kg。该抗蛇毒血清对中东金蝎毒液显示出中和作用。1毫升粗尾蝎抗蛇毒血清可中和464个中东金蝎半数致死剂量和940个粗尾蝎半数致死剂量的毒液（对小鼠而言）。蛋白质印迹法显示了与毒液的免疫反应性。单价抗蛇毒血清对蝎毒具有免疫活性和中和能力。本研究表明，由雷菲克·赛达姆卫生中心生产的抗蛇毒血清可用于治疗土耳其的中东金蝎蜇伤。
