SIRT6 和 NMNAT2 的下调与增殖性糖尿病性视网膜病变有关。

Downregulation of SIRT6 and NMNAT2 is associated with proliferative diabetic retinopathy.

机构信息

State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangdong Provincial Key Laboratory of Ophthalmology and Visual Science, Guangzhou, China.

出版信息

Mol Vis. 2023 Oct 2;29:160-168. eCollection 2023.

PMID:38222451

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10784219/

Abstract

PURPOSE

To determine the expression levels of and in diabetic retinopathy (DR).

METHODS

We obtained peripheral blood mononuclear cells (PBMCs) and vitreous samples from 77 patients with type 2 diabetes mellitus: 52 with DR and 25 without DR, and 27 healthy control subjects. Western blot analysis and qRT-PCR were performed to evaluate the expression of and in their PBMCs. The levels of , , and in the vitreous fluid were determined by ELISA. Immunohistochemistry was performed to detect the expression of and 2 in proliferative DR (PDR) and the control subjects.

RESULTS

The expression of and was markedly downregulated in DR patients, which was negatively correlated with the increased expression of and . Additionally, we observed decreased expression of and in the fibrovascular membranes of PDR patients.

CONCLUSIONS

The downregulated expression of and in PDR patients reveals a potential pathogenic association; more extended studies could verify them as potential therapeutic targets.

摘要

目的

检测糖尿病视网膜病变（DR）患者中和的表达水平。

方法

收集 77 例 2 型糖尿病患者（52 例 DR 和 25 例非 DR）和 27 例健康对照者的外周血单个核细胞（PBMC）和玻璃体样本。采用 Western blot 分析和 qRT-PCR 检测 PBMC 中和的表达。采用 ELISA 法检测玻璃体中、和的水平。免疫组化法检测增生性 DR（PDR）患者和对照组中和 2 的表达。