Gui Lin-Sheng, Zhang Ya-Ran, Liu Gui-Yao, Zan Lin-Sen
College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China.
Institute of Soil and Water Conservation, Chinese Academy of Science and Ministry of Water Resource, Yangling 712100, China.
Int J Mol Sci. 2015 Jan 22;16(2):2458-71. doi: 10.3390/ijms16022458.
Silent information regulator 2 (SIRT2) is a member of the sirtuin family of class III NAD (nicotinamide adenine dinucleotide)-dependent protein deacetylases and may regulate senescence, metabolism and apoptosis. The aims of this study were to investigate whether the SIRT2 gene could be used as a candidate gene in the breeding of Qinchuan cattle. Real-time polymerase chain reaction (RT-PCR) results showed that among all types of tissue that were analyzed, the highest mRNA expression levels of the gene were found in subcutaneous fat. DNA sequencing of 468 individual Qinchuan cattle identified two novel, single nucleotide polymorphisms (g.19501 C > T and g.19518 C > T) in the 3' untranslated region (3'UTR) of the SIRT2 gene. The frequencies of SNP g.19501 C > T and g.19518 C > T were in Hardy-Weinberg disequilibrium in all the samples (chi-square test, χ2 < χ0.052). An association analysis showed that the two loci were significantly correlated with some body size traits and the H2H2 (-CT-CT-) diplotypes performed better than other combinations. These results indicated that the variations in the SIRT2 gene and their corresponding genotypes may be considered as molecular markers for economic traits in cattle breeding.
沉默信息调节因子2（SIRT2）是III类烟酰胺腺嘌呤二核苷酸（NAD）依赖性蛋白脱乙酰酶的沉默调节蛋白家族成员，可能调节衰老、代谢和细胞凋亡。本研究的目的是调查SIRT2基因是否可作为秦川牛育种的候选基因。实时聚合酶链反应（RT-PCR）结果显示，在所分析的所有组织类型中，该基因的mRNA表达水平在皮下脂肪中最高。对468头秦川牛个体进行DNA测序，在SIRT2基因的3'非翻译区（3'UTR）中鉴定出两个新的单核苷酸多态性（g.19501 C>T和g.19518 C>T）。SNP g.19501 C>T和g.19518 C>T的频率在所有样本中均处于哈迪-温伯格不平衡状态（卡方检验，χ2<χ0.052）。关联分析表明，这两个位点与一些体尺性状显著相关，并且H2H2（-CT-CT-）单倍型组合的表现优于其他组合。这些结果表明，SIRT2基因的变异及其相应的基因型可被视为牛育种中经济性状的分子标记。
