Konska G, Guillot J, Dusser M, Damez M, Botton B
Department of Botany and Microbiology, Faculty of Pharmaceutical Sciences, University of Clermont, Clermont-Ferrand, France.
J Biochem. 1994 Sep;116(3):519-23. doi: 10.1093/oxfordjournals.jbchem.a124555.
A hemagglutinating and hemolytic lectin (PSL) has been isolated from carpophores of the parasitic mushroom Laetiporus sulfureus by affinity chromatography on Sepharose. Its molecular weight, as determined both by gel filtration and by electrophoresis in non-denaturing conditions, is about 190,000 and its structure is tetrameric, with two distinct types of subunits (about 60,000 and 36,000). It appeared homogeneous on HPLC gel filtration but exhibited microheterogeneity on isoelectric focusing. Hapten inhibition assay indicated that the Laetiporus lectin is specific for N-acetyllactosamine residues and that hemagglutinating and hemolysis activities are supported by the same site.
通过琼脂糖凝胶亲和层析从寄生蘑菇硫黄多孔菌的子实体中分离出一种具有血凝和溶血活性的凝集素（PSL）。通过凝胶过滤和非变性条件下的电泳测定，其分子量约为190,000，结构为四聚体，有两种不同类型的亚基（约60,000和36,000）。在高效液相色谱凝胶过滤中它表现为均一，但在等电聚焦时呈现微不均一性。半抗原抑制试验表明，硫黄多孔菌凝集素对N-乙酰乳糖胺残基具有特异性，并且血凝和溶血活性由同一位点支持。
