Parasitological Laboratory, Bulevar Oslobodjenja, Belgrade, Serbia.
Clin Microbiol Infect. 2009 Dec;15(12):1173-6. doi: 10.1111/j.1469-0691.2009.02768.x. Epub 2009 Apr 18.
A retrospective epidemiological and diagnostic study of visceral leishmaniasis (VL) was carried out during the period 2001-2007 and included patients suspected of VL who had been diagnosed at the Parasitological Laboratory at the Institute for Infectious and Tropical Diseases, Belgrade. Diagnosis of VL was confirmed by microscopic examination of Giemsa-stained bone marrow (BM) smears. BM smears from 134 patients were examined; 22 cases of VL were diagnosed, the majority of which involved individuals who had been on holiday at the Montenegrian sea coast. The sensitivity of the initial BM smears was inadequate; this required the application of a serological test, adapted for routine use, for the diagnosis of VL.
一项回顾性流行病学和诊断研究于 2001-2007 年进行,包括在贝尔格莱德传染病和热带病研究所寄生虫学实验室诊断为内脏利什曼病(VL)的疑似患者。VL 的诊断通过吉姆萨染色骨髓(BM)涂片的显微镜检查得到确认。共检查了 134 例 BM 涂片,诊断出 22 例 VL,其中大多数涉及在黑山沿海度假的个体。初始 BM 涂片的敏感性不足;这需要应用一种适应常规使用的血清学检测方法来诊断 VL。