Lee Y J, Curetty L, Corry P M
Department of Radiation Oncology, William Beaumont Hospital, Royal Oak, Michigan 48073.
J Cell Physiol. 1991 Oct;149(1):77-87. doi: 10.1002/jcp.1041490111.
Since both heat and sodium arsenite induce thermotolerance, we investigated the differences in synthesis and redistribution of stress proteins induced by these agents in Chinese hamster ovary cells. Five major heat shock proteins (HSPs; Mr 110, 87, 70, 28, and 8.5 kDa) were preferentially synthesized after heat for 10 min at 45.5 degrees C, whereas four major HSPs (Mr 110, 87, 70, and 28 kDa) and one stress protein (33.3 kDa) were preferentially synthesized after treatment with 100 microM sodium arsenite (ARS) for 1 hr. Two HSP families (HSP70a,b,c, and HSP28a,b,c) preferentially relocalized in the nucleus after heat shock. In contrast, only HSP70b redistributed into the nucleus after ARS treatment. Furthermore, the kinetics of synthesis of each member of HSP70 and HSP28 families and their redistribution were different after these treatments. The maximum rates of synthesis of HSP70 and HSP28 families, except HSP28c, were 6-9 hr after heat shock, whereas those of HSP70b and HSP28b,c were 0-2 hr after ARS treatment. In addition, the maximum rates of redistribution of HSP70 and HSP28 families occurred 3-6 hr after heat shock, whereas that of HSP70b occurred immediately after ARS treatment. The degree of redistribution of HSP70b after ARS treatment was significantly less than that after heat treatment. These results suggest that heat treatment but not sodium arsenite treatment stimulates the entry of HSP70 and HSP28 families into the nucleus.
由于热和亚砷酸钠均可诱导热耐受性，我们研究了这些试剂在中国仓鼠卵巢细胞中诱导的应激蛋白的合成和重新分布的差异。在45.5℃加热10分钟后，优先合成了五种主要的热休克蛋白（HSPs；分子量分别为110、87、70、28和8.5 kDa），而在用100 microM亚砷酸钠（ARS）处理1小时后，优先合成了四种主要的HSPs（分子量分别为110、87、70和28 kDa）和一种应激蛋白（33.3 kDa）。两个HSP家族（HSP70a、b、c和HSP28a、b、c）在热休克后优先重新定位于细胞核。相比之下，ARS处理后只有HSP70b重新分布到细胞核中。此外，这些处理后，HSP70和HSP28家族各成员的合成动力学及其重新分布情况有所不同。热休克后6-9小时，HSP70和HSP28家族（除HSP28c外）的合成速率达到最大值，而ARS处理后0-2小时，HSP70b和HSP28b、c的合成速率达到最大值。另外，热休克后3-6小时，HSP70和HSP28家族的重新分布速率达到最大值，而ARS处理后HSP70b的重新分布速率立即达到最大值。ARS处理后HSP70b的重新分布程度明显低于热处理后的程度。这些结果表明，热处理而非亚砷酸钠处理会刺激HSP70和HSP28家族进入细胞核。
