Mason Rosemarie D, De Rose Robert, Kent Stephen J
Department of Microbiology and Immunology, University of Melbourne 3010, Australia.
Virology. 2009 Jun 5;388(2):315-23. doi: 10.1016/j.virol.2009.03.020. Epub 2009 Apr 24.
Cytotoxic T lymphocyte responses to conserved proteins such as Gag within HIV- or SIV-infected hosts can facilitate partial control of viremia. However, the utility of targeting variable viral proteins by CTL responses is unclear. We studied CTL responses to regulatory and accessory proteins of SIV in pigtail macaques. The regulatory and accessory proteins were the most commonly targeted proteins by CTL responses from pigtail macaques. We identified 2 novel Tat-specific CTL responses that were both restricted by the Mane-A10 allele. Viral escape at one of the Tat epitopes, KSA10, was slower in comparison to another Tat epitope KVA10. The kinetics of escape of the KSA10 Tat epitope were more similar to an immunodominant KP9 Gag epitope also restricted by Mane-A10. Our results suggest that some regulatory or accessory CTL epitopes may be useful targets for vaccination against HIV.
在感染HIV或SIV的宿主体内，细胞毒性T淋巴细胞对诸如Gag等保守蛋白的反应可促进对病毒血症的部分控制。然而，CTL反应靶向可变病毒蛋白的效用尚不清楚。我们研究了猪尾猕猴中针对SIV调节蛋白和辅助蛋白的CTL反应。调节蛋白和辅助蛋白是猪尾猕猴CTL反应最常靶向的蛋白。我们鉴定出2种新的Tat特异性CTL反应，它们均受Mane - A10等位基因限制。与另一个Tat表位KVA10相比，Tat表位之一KSA10处的病毒逃逸较慢。KSA10 Tat表位的逃逸动力学更类似于同样受Mane - A10限制的免疫显性KP9 Gag表位。我们的结果表明，一些调节或辅助CTL表位可能是抗HIV疫苗接种的有用靶点。
