Jones D J, Nguyen K T, McLeish M J, Crankshaw D P, Morgan D J
Department of Anaesthetics, Royal Melbourne Hospital, Victoria, Australia.
J Chromatogr B Biomed Appl. 1996 Jan 12;675(1):174-9. doi: 10.1016/0378-4347(95)00331-2.
A method for the determination of R-(+)- and (S)-(-)-isomers of thiopentone in plasma was developed. Following liquid-liquid extraction, the separation of enantiomers of thiopentone and the internal standard (racemic ketamine) was achieved by high-performance liquid chromatography on an alpha1-acid glycoprotein (AGP) column with ultraviolet detection at 280 nm. The mobile phase consisted of 20 mM KH2PO4 buffer-2-propanol-methanol (93.5:5.0:1.5) at pH 5.0. The flow-rate was 0.9 ml/min. The limit of quantification for each isomer was approximately 10 ng/ml. The assay is suitable for pharmacokinetic studies of (R)-(+)- and (S)-(-)-isomers of thiopentone, following usual bolus intravenous clinical doses of the racemic drug.
建立了一种测定血浆中硫喷妥R-(+)-和(S)-(-)-异构体的方法。液-液萃取后，硫喷妥对映体与内标（外消旋氯胺酮）通过在α1-酸性糖蛋白（AGP）柱上的高效液相色谱法进行分离，在280 nm处进行紫外检测。流动相由pH 5.0的20 mM KH2PO4缓冲液-2-丙醇-甲醇（93.5:5.0:1.5）组成。流速为0.9 ml/min。每种异构体的定量限约为10 ng/ml。该测定法适用于在给予外消旋药物常规静脉推注临床剂量后，对硫喷妥R-(+)-和(S)-(-)-异构体进行药代动力学研究。
