Hermansson J, Eriksson M, Nyquist O
J Chromatogr. 1984 Dec 12;336(2):321-8. doi: 10.1016/s0378-4347(00)85155-5.
The direct resolution and quantitation of (R)- and (S)-disopyramide, isolated from human plasma, was accomplished using a chiral alpha 1-acid glycoprotein column. A LiChrosorb RP-2 column (50 X 3.0 mm I.D.) was used as a precolumn. Phosphate buffer, pH 6.20, containing 2-propanol and N,N-dimethyloctylamine was used as mobile phase. The precision of the determination of (R)- and (S)-disopyramide in human plasma, expressed as the relative standard deviation, was 1.8% and 3.3% for (R)- and (S)-disopyramide, respectively, at a drug level of 0.5 micrograms/ml. In two subjects who received a single capsule of racemic disopyramide (150 mg), the plasma levels of the (R) isomer were about half those of the (S) isomer. The half-lives of (R)- and (S)-disopyramide were similar.
采用手性α1-酸性糖蛋白柱对从人血浆中分离出的（R）-和（S）-丙吡胺进行直接拆分和定量。使用LiChrosorb RP - 2柱（50×3.0 mm内径）作为预柱。以含2 - 丙醇和N，N - 二甲基辛胺的pH 6.20磷酸盐缓冲液作为流动相。在药物浓度为0.5微克/毫升时，人血浆中（R）-和（S）-丙吡胺测定的精密度，以相对标准偏差表示，（R）-丙吡胺为1.8%，（S）-丙吡胺为3.3%。在两名服用消旋丙吡胺单胶囊（150毫克）的受试者中，（R）异构体的血浆水平约为（S）异构体的一半。（R）-和（S）-丙吡胺的半衰期相似。
