烟草花叶病毒在油/水界面的自组装。

Self-assembly of tobacco mosaic virus at oil/water interfaces.

作者信息

He Jinbo, Niu Zhongwei, Tangirala Ravisubhash, Wang Jia-Yu, Wei Xinyu, Kaur Gagandeep, Wang Qian, Jutz Günther, Böker Alexander, Lee Byeongdu, Pingali Sai Venkatesh, Thiyagarajan Pappannan, Emrick Todd, Russell Thomas P

机构信息

Department of Polymer Science & Engineering, University of Massachusetts, Amherst, Massachusetts 01003, USA.

出版信息

Langmuir. 2009 May 5;25(9):4979-87. doi: 10.1021/la803533n.

DOI:10.1021/la803533n

PMID:19397351

Abstract

The oil/water interfacial assembly of tobacco mosaic virus (TMV) has been studied in situ by tensiometry and small-angle X-ray and neutron scattering (SAXS and SANS). TMV showed different orientations at the perfluorodecalin/water interface, depending on the initial TMV concentration in the aqueous phase. At low TMV concentration, the rods oriented parallel to the interface, mediating the interfacial interactions at the greatest extent per particle. At high TMV concentrations, the rods were oriented normal to the interface, mediating the interfacial interactions and also neutralizing inter-rod electrostatic repulsion. We found that the inter-rod repulsive forces between TMVs dominated the in-plane packing, which was strongly affected by the ionic strength and the bulk solution but not by the pH in the range of pH = 6-8.

摘要

通过张力测量法以及小角X射线和中子散射（SAXS和SANS）对烟草花叶病毒（TMV）的油/水界面组装进行了原位研究。TMV在全氟萘烷/水界面呈现出不同的取向，这取决于水相中初始TMV的浓度。在低TMV浓度下，棒状粒子平行于界面取向，每个粒子在最大程度上介导界面相互作用。在高TMV浓度下，棒状粒子垂直于界面取向，介导界面相互作用并中和棒间的静电排斥。我们发现TMV之间的棒间排斥力主导了平面内堆积，这受到离子强度和本体溶液的强烈影响，但在pH = 6 - 8范围内不受pH值的影响。