Yurimoto Hiroya, Sakai Yasuyoshi
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan.
Biotechnol Appl Biochem. 2009 May 6;53(Pt 2):85-92. doi: 10.1042/BA20090030.
Methylotrophic yeasts can obtain all of their required carbon and energy from methanol, a promising feedstock for biotechnological and chemical processes and a potential replacement for coal and petroleum. When these yeast cells are cultured in the presence of methanol, the enzymes involved in methanol metabolism are massively produced, indicating that the gene promoters of these enzymes are strong methanol-inducible promoters. Using these promoters, high-level heterologous gene expression systems have been developed in several methylotrophic yeast strains, including Pichia pastoris, Hansenula polymorpha, Pichia methanolica and Candida boidinii. In the present minireview we describe recent insights into the molecular basis of methanol-inducible gene expression in C. boidinii. In addition, the utility of C. boidinii gene expression systems for the production of various enzymes is also reviewed.
甲基营养型酵母能够从甲醇中获取其所需的全部碳源和能量。甲醇是生物技术和化学过程中一种很有前景的原料，也是煤炭和石油的潜在替代品。当这些酵母细胞在甲醇存在的情况下进行培养时，参与甲醇代谢的酶会大量产生，这表明这些酶的基因启动子是强甲醇诱导型启动子。利用这些启动子，已经在几种甲基营养型酵母菌株中开发出了高水平的异源基因表达系统，包括巴斯德毕赤酵母、多形汉逊酵母、甲醇毕赤酵母和博伊丁假丝酵母。在本综述中，我们描述了对博伊丁假丝酵母中甲醇诱导型基因表达分子基础的最新见解。此外，还综述了博伊丁假丝酵母基因表达系统在生产各种酶方面的应用。
