甲基营养型酵母博伊丁假丝酵母中的基因标签诱变
Gene-tagging mutagenesis in the methylotrophic yeast Candida boidinii.
作者信息
Sasano Yu, Yurimoto Hiroya, Sakai Yasuyoshi
机构信息
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa-Oiwake, Kyoto, Japan.
出版信息
J Biosci Bioeng. 2007 Jul;104(1):86-9. doi: 10.1263/jbb.104.86.
PMID:17697989
Abstract
A gene-tagging mutagenesis method by random integration of linear DNA fragments was developed and used in the methylotrophic yeast Candida boidinii to isolate mutants defective in methanol-inducible gene expression. A large number of mutants were obtained, indicating that this method is a powerful tool for random mutagenesis in C. boidinii.
摘要
通过线性DNA片段的随机整合开发了一种基因标签诱变方法，并将其用于甲基营养型酵母博伊丁假丝酵母中，以分离甲醇诱导型基因表达缺陷的突变体。获得了大量突变体，表明该方法是博伊丁假丝酵母中进行随机诱变的有力工具。
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