Li Y, Lim L-T, Kakuda Y
Deparment of Food Science, Univ. of Guelph, Guelph, Ontario, Canada.
J Food Sci. 2009 Apr;74(3):C233-40. doi: 10.1111/j.1750-3841.2009.01093.x.
In this study, a method was developed for continuous electrospinning of ultrafine corn zein protein fibers with diameters ranging from 150 to 600 nm. Fiber-forming solutions with various zein concentrations (10% to 30%, w/w) and aqueous ethanol concentrations (60% to 90%, w/w) were electrospun at 15 and 20 kV. Scanning electron microscopy results showed that the morphology of zein fibers was affected by aqueous ethanol concentration, zein concentration, and the applied voltage. The optimal condition for forming bead-less fibers was found to be 20% protein, 70% alcohol, and 15 kV. The zein fibers resisted solubilization in water, although swelling and plasticization were apparent after the water treatment. The efficacy of zein fibers was tested for stabilization of a green tea polyphenol, (-)-epigallocatechin gallate (EGCG), by incorporating the EGCG in zein fiber-forming solutions. Freshly spun fibers were less effective at immobilizing the EGCG upon immersion in water (82% recovery) as compared to fibers that were aged at 0% relative humidity for at least 1 d (>98% recovery) before water immersion. Fourier transform infrared spectroscopy studies demonstrated that hydrogen bonding, hydrophobic interactions, and physical encapsulation are the major contributors to the stabilization of EGCG in zein fibers in water.
在本研究中，开发了一种用于连续静电纺丝制备直径范围为150至600 nm的超细玉米醇溶蛋白纤维的方法。将具有不同玉米醇溶蛋白浓度（10%至30%，w/w）和乙醇水溶液浓度（60%至90%，w/w）的成纤溶液在15 kV和20 kV下进行静电纺丝。扫描电子显微镜结果表明，玉米醇溶蛋白纤维的形态受乙醇水溶液浓度、玉米醇溶蛋白浓度和施加电压的影响。发现形成无珠纤维的最佳条件是20%的蛋白质、70%的乙醇和15 kV。玉米醇溶蛋白纤维在水中抗溶解，尽管水处理后明显出现溶胀和增塑现象。通过将（-）-表没食子儿茶素没食子酸酯（EGCG）掺入玉米醇溶蛋白成纤溶液中，测试了玉米醇溶蛋白纤维对绿茶多酚EGCG的稳定效果。与在0%相对湿度下老化至少1天（回收率>98%）后再浸入水中的纤维相比，新鲜纺制的纤维在浸入水中时固定EGCG的效果较差（回收率82%）。傅里叶变换红外光谱研究表明，氢键、疏水相互作用和物理包封是EGCG在水中的玉米醇溶蛋白纤维中稳定化的主要因素。
