Pastel-Levy C, Flotte T J, Preffer F, Ware A, Graeme-Cook F, Bell D A, Colvin R B
Department of Pathology, Massachusetts General Hospital, Boston 02114.
J Cutan Pathol. 1991 Aug;18(4):279-83. doi: 10.1111/j.1600-0560.1991.tb01236.x.
The distinction of mycosis fungoides from benign inflammatory lesions is sometimes difficult by conventional histological techniques. Aneuploidy, a feature often associated with malignant tumors, can be assessed even in tissue routinely processed in paraffin using the flow cytometric technique of Hedley and associates. In many tumor systems, there are significant diploid clones. We have evaluated the flow cytometric DNA ploidy of paraffin-embedded tissue in the diagnosis and prognosis of mycosis fungoides (MF). We studied 22 cases of MF and 10 control cases of inflammatory skin lesions with epidermal involvement. Aneuploidy was found in 27% of the MF cases, but in none of the controls (ED). Aneuploid features were seen in 23% of tissues from early stage disease. Aneuploidy did not correlate with atypia, epidermotropism, or number of mitoses. There was a trend towards showing adverse outcome in those patients with aneuploid lesions. The detection of aneuploidy might be helpful for early diagnosis of MF.
通过传统组织学技术有时很难区分蕈样肉芽肿与良性炎症性病变。非整倍体是常与恶性肿瘤相关的一个特征，即使在石蜡常规处理的组织中，也可使用Hedley及其同事的流式细胞术进行评估。在许多肿瘤系统中，存在显著的二倍体克隆。我们评估了石蜡包埋组织的流式细胞术DNA倍性在蕈样肉芽肿（MF）诊断和预后中的作用。我们研究了22例MF病例和10例有表皮受累的炎症性皮肤病变对照病例。27%的MF病例中发现非整倍体，但对照病例（ED）中均未发现。疾病早期组织中有23%可见非整倍体特征。非整倍体与异型性、亲表皮性或有丝分裂数无关。有非整倍体病变的患者有显示不良预后的趋势。非整倍体的检测可能有助于MF的早期诊断。
