von Roenn J H, Kheir S M, Wolter J M, Coon J S
Cancer Res. 1986 Jun;46(6):3192-5.
DNA ploidy of melanocytic skin tumors from 87 patients (53 primary melanomas, 34 nevi) was determined by flow cytometry from routinely prepared paraffin blocks. Ploidy data correlated strongly with conventional morphological parameters. Only 1 of 34 nevi, but 13 of 53 melanomas were aneuploid. Among the melanomas, none of 21 levels I-III melanomas was aneuploid, but 13 of 32 levels IV and V melanomas were aneuploid. There was also a significant correlation between increasing Breslow thickness and the presence of DNA aneuploidy. For 33 melanoma patients with over 2 yr of follow-up (average, 7.1 yr), only 4 of 23 diploid tumors have recurred, but 9 of 10 aneuploid tumors have recurred. We conclude that the biological potential of melanocytic skin tumors is strongly linked to DNA aneuploidy. Since this parameter can be conveniently determined from paraffin blocks, determination of ploidy abnormalities in these tumors may be clinically useful.
通过流式细胞术对87例患者（53例原发性黑色素瘤、34例痣）的皮肤黑素细胞肿瘤进行DNA倍体分析，样本取自常规制备的石蜡块。倍体数据与传统形态学参数密切相关。34例痣中只有1例为非整倍体，而53例黑色素瘤中有13例为非整倍体。在黑色素瘤中，21例I - III期黑色素瘤均无非整倍体，但32例IV期和V期黑色素瘤中有13例为非整倍体。Breslow厚度增加与DNA非整倍体的存在之间也存在显著相关性。对33例随访超过2年（平均7.1年）的黑色素瘤患者进行分析，23例二倍体肿瘤中只有4例复发，但10例非整倍体肿瘤中有9例复发。我们得出结论，皮肤黑素细胞肿瘤的生物学潜能与DNA非整倍体密切相关。由于该参数可方便地从石蜡块中测定，因此测定这些肿瘤的倍体异常可能具有临床应用价值。
