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Sgt1的二聚化受到蛋白激酶CK2介导的Ser361位点磷酸化的负调控。

Sgt1 dimerization is negatively regulated by protein kinase CK2-mediated phosphorylation at Ser361.

作者信息

Bansal Parmil K, Mishra Ashutosh, High Anthony A, Abdulle Rashid, Kitagawa Katsumi

机构信息

Department of Molecular Pharmacology, Hartwell Center for Bioinformatics and Biotechnology, St. Jude Children's Research Hospital, Memphis, Tennessee 38105-3678, USA.

出版信息

J Biol Chem. 2009 Jul 10;284(28):18692-8. doi: 10.1074/jbc.M109.012732. Epub 2009 Apr 27.

DOI:10.1074/jbc.M109.012732
PMID:19398558
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2707205/
Abstract

The kinetochore, which consists of centromere DNA and structural proteins, is essential for proper chromosome segregation in eukaryotes. In budding yeast, Sgt1 and Hsp90 are required for the binding of Skp1 to Ctf13 (a component of the core kinetochore complex CBF3) and therefore for the assembly of CBF3. We have previously shown that Sgt1 dimerization is important for this kinetochore assembly mechanism. In this study, we report that protein kinase CK2 phosphorylates Ser(361) on Sgt1, and this phosphorylation inhibits Sgt1 dimerization.

摘要

动粒由着丝粒DNA和结构蛋白组成，对真核生物中染色体的正确分离至关重要。在芽殖酵母中，Sgt1和Hsp90是Skp1与Ctf13（核心动粒复合体CBF3的一个组分）结合所必需的，因此也是CBF3组装所必需的。我们之前已经表明，Sgt1二聚化对这种动粒组装机制很重要。在本研究中，我们报告蛋白激酶CK2使Sgt1上的Ser(361)磷酸化，这种磷酸化抑制Sgt1二聚化。

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本文引用的文献

1
Sgt1 dimerization is required for yeast kinetochore assembly.酵母动粒组装需要 Sgt1 二聚化。
J Biol Chem. 2009 Feb 6;284(6):3586-92. doi: 10.1074/jbc.M806281200. Epub 2008 Dec 10.
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Molecular architecture of the kinetochore-microtubule interface.动粒-微管界面的分子结构
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TPR-Mediated self-association of plant SGT1.TPR介导的植物SGT1自缔合
Biochemistry. 2007 Oct 9;46(40):11331-41. doi: 10.1021/bi700735t. Epub 2007 Sep 18.
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Targeting mitosis for anti-cancer therapy.靶向有丝分裂进行抗癌治疗。
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Structures and functions of yeast kinetochore complexes.酵母动粒复合体的结构与功能
Annu Rev Biochem. 2007;76:563-91. doi: 10.1146/annurev.biochem.76.052705.160607.
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Aneuploidy acts both oncogenically and as a tumor suppressor.非整倍体既具有致癌作用，也可作为一种肿瘤抑制因子发挥作用。
Cancer Cell. 2007 Jan;11(1):25-36. doi: 10.1016/j.ccr.2006.12.003. Epub 2006 Dec 28.
7
Sgt1p is a unique co-chaperone that acts as a client adaptor to link Hsp90 to Skp1p.Sgt1p是一种独特的共伴侣蛋白，作为一种客户适配器，将热休克蛋白90（Hsp90）与Skp1p连接起来。
J Biol Chem. 2006 Nov 3;281(44):33739-48. doi: 10.1074/jbc.M603847200. Epub 2006 Aug 31.
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17-AAG, an Hsp90 inhibitor, causes kinetochore defects: a novel mechanism by which 17-AAG inhibits cell proliferation.17-烯丙基氨基-17-去甲氧基格尔德霉素（17-AAG），一种热休克蛋白90（Hsp90）抑制剂，可导致动粒缺陷：这是17-AAG抑制细胞增殖的一种新机制。
Oncogene. 2006 Jul 13;25(30):4133-46. doi: 10.1038/sj.onc.1209461. Epub 2006 Feb 27.
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A global view of CK2 function and regulation.CK2功能与调控的全局观。
Mol Cell Biochem. 2005 Jun;274(1-2):163-70. doi: 10.1007/s11010-005-2945-z.
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Different properties of four molecular forms of protein kinase CK2 from Saccharomyces cerevisiae.来自酿酒酵母的蛋白激酶CK2四种分子形式的不同特性。
Acta Biochim Pol. 2005;52(4):947-51. Epub 2005 Oct 25.