系统性红斑狼疮中雌激素受体α基因多态性与细胞因子基因表达的关联
Association of estrogen receptor alpha gene polymorphisms with cytokine genes expression in systemic lupus erythematosus.
作者信息
Lu Zhi-Ming, Wang Zi-E, Liu Yi-Qing, Wu Chun-Xiao, Wang Chang-Yin, Zhang Bing-Chang, Shao Song, Jiao Yu-Lian, Che Zhi-Xiang, Chen Zi-Jiang, Zhao Yue-Ran
机构信息
Department of Laboratory Medicine, Shandong Provincial Hospital, Shandong University, Jinan 250021, People's Republic of China.
出版信息
Croat Med J. 2009 Apr;50(2):117-23. doi: 10.3325/cmj.2009.50.117.
AIM
To analyze the association of estrogen receptor alpha (OR alpha) gene polymorphisms with cytokine genes expression in patients with systemic lupus erythematosus (SLE) and controls.
METHODS
Genomic DNA was extracted and polymorphisms of XbaI, Ukrainian (XX, Xx, or xx genotype) and PvuII (PP, Pp, or pp) in intron 1 of OR alpha gene were detected by polymerase chain reaction-restriction fragment length polymorphisms (PCR-RFLP) method. The messenger RNA (mRNA) levels of interleukin (IL)-10, IL-4, interferon (IFN)-gamma, and IL-2 were assessed by semiquantitative reverse transcription polymerase chain reaction (RT-PCR).
RESULTS
In patients with SLE with PpXx genotype, IL-10 and IL-4 mRNA expression was higher (P < 0.001 and P = 0.013, respectively), while in patients with SLE with Ppxx genotype IFN-gamma and IL-2 mRNA expression was lower than in controls (P < 0.001). There was no significant difference in mRNA expression of 4 cytokines among controls with various genotypes.
CONCLUSION
OR alpha gene polymorphism may be associated with the expression of IL-10, IL-4, IL-2, and IFN-gamma in patients with SLE.
目的
分析雌激素受体α（ERα）基因多态性与系统性红斑狼疮（SLE）患者及对照者细胞因子基因表达的相关性。
方法
提取基因组DNA，采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法检测ERα基因第1内含子中XbaI、乌兹别克（XX、Xx或xx基因型）和PvuII（PP、Pp或pp）的多态性。通过半定量逆转录聚合酶链反应（RT-PCR）评估白细胞介素（IL）-10、IL-4、干扰素（IFN）-γ和IL-2的信使核糖核酸（mRNA）水平。
结果
SLE患者中，PpXx基因型患者的IL-10和IL-4 mRNA表达较高（分别为P < 0.001和P = 0.013），而Ppxx基因型的SLE患者中IFN-γ和IL-2 mRNA表达低于对照者（P < 0.001）。不同基因型对照者中4种细胞因子的mRNA表达无显著差异。
结论
ERα基因多态性可能与SLE患者IL-10、IL-4、IL-2和IFN-γ的表达有关。
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