Chu Yueng-Hsiang, Wu Chin-Chen, Wu Ching-Hsiang, Wang Hsing-Won
Department of Otolaryngology-Head and Neck Surgery, Tri-Service General Hospital, Taipei, Taiwan, Republic of China.
Am J Rhinol Allergy. 2009 Mar-Apr;23(2):162-6. doi: 10.2500/ajra.2009.23.3288.
Cooling may induce nasal obstruction. In our previous study, we showed low temperature induced a rapid relaxation of isolated human nasal mucosa and it was independent of vasoconstriction. The aim of this study was to elucidate the mechanism responsible for such findings.
Nasal mucosa strips were prepared from inferior turbinate samples. Decellularization of human nasal mucosa was achieved by treatment with sodium dodecyl sulfate 0.1% for 15 hours at 37 degrees C in a shaking water bath. Then, the effect of cooling (37-10 degrees C) on the isometric tension change of decellularized nasal mucosa was evaluated. In addition, the presence of elastic fibers within the nasal mucosa was identified in both histological section and scanning electron microscope.
Results indicated cooling induced a relaxation response of isolated decellularized human nasal mucosa and it was similar to that of intact nasal mucosa. The elastic fibers formed in wavy lines and were distributed throughout the submucosal layer.
Cooling induced a similar relaxation response both in the absence or in the presence of cellular components in isolated human nasal mucosa, suggesting that this response is mediated by the abundant extracellular matrix.
冷却可能会导致鼻塞。在我们之前的研究中，我们发现低温会使分离的人鼻黏膜迅速松弛，且这一过程与血管收缩无关。本研究的目的是阐明导致这些发现的机制。
从下鼻甲样本制备鼻黏膜条。通过在37℃的振荡水浴中用0.1%的十二烷基硫酸钠处理15小时，实现人鼻黏膜的脱细胞处理。然后，评估冷却（37℃至10℃）对脱细胞鼻黏膜等长张力变化的影响。此外，在组织学切片和扫描电子显微镜下鉴定鼻黏膜内弹性纤维的存在。
结果表明，冷却会引起分离的脱细胞人鼻黏膜的松弛反应，且与完整鼻黏膜的反应相似。弹性纤维呈波浪状形成，并分布于整个黏膜下层。
在分离的人鼻黏膜中，无论有无细胞成分，冷却都会引起类似的松弛反应，这表明这种反应是由丰富的细胞外基质介导的。
