大鼠脑中细胞色素P - 450及芳烃羟化酶活性的出现情况。
On the occurrence of cytochrome P-450 and aryl hydrocarbon hydroxylase activity in rat brain.
作者信息
Cohn J A, Alvares A P, Kappas A
出版信息
J Exp Med. 1977 Jun 1;145(6):1607-11. doi: 10.1084/jem.145.6.1607.
Abstract
The difference spectra of the carbon monoxide-complex of dithionite-reduced rat brain microsomes, compared with both reduced microsomes, alone, and the carbon monoxide-complex of oxidized microsomes, indicate the presence of small amounts of cytochrome P-450 in brain. As in liver, cytochrome P-450 in brain is degraded in vitro to its inactive form, cytochrome P-420 by methylmercury chloride. Aryl hydrocarbon hydroxylase activity is also present in rat brain microsomes and, at lower specific activity, in brain homogenates. This carcinogen metabolizing activity is increased four-fold in rats pretreated with 3-methylcholanthrene.
摘要
连二亚硫酸盐还原的大鼠脑微粒体与一氧化碳形成的复合物的差示光谱，与单独的还原微粒体以及氧化微粒体与一氧化碳形成的复合物相比，表明脑中存在少量细胞色素P-450。与肝脏一样，脑中的细胞色素P-450在体外会被甲基氯化汞降解为无活性形式的细胞色素P-420。大鼠脑微粒体中也存在芳烃羟化酶活性，在脑匀浆中的比活性较低。在用3-甲基胆蒽预处理的大鼠中，这种致癌物代谢活性增加了四倍。
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