Whyte S K, Secombes C J, Chappell L H
Department of Zoology, University of Aberdeen, UK.
J Helminthol. 1991 Sep;65(3):169-78. doi: 10.1017/s0022149x0001066x.
The infectivity of Diplostomum spathaceum (Digenea: Trematoda) cercariae to rainbow trout and the efficacy of the diplostomule migration to the lens following different routes of administration was examined. The optimum age of infectivity for cercariae was between 0-5 h after liberation from the snail and for intraperitoneally injected diplostomules, 5 h post-transformation in vitro through fish skin. After exposure of the entire fish body or head to cercariae, metacercariae first appeared in the lens at 5 h and their numbers gradually increased until 22 h. Following exposure of the tail region of rainbow trout to cercariae, metacercariae first appeared in the lens at 14 h. Significantly more metacercariae established in the lens of fish following exposure of the fish head compared with the tail region; 40% of penetrating cercariae reached the lens of fish following exposure of the head or entire body, 20% of cercariae or diplostomules injected either intraperitoneally, intramuscularly or intracardially reached the lens while only 5% of cercariae established as metacercariae following exposure of the tail region.
研究了卵形双腔吸虫（复殖目：吸虫纲）尾蚴对虹鳟的感染性以及不同给药途径后双穴吸虫蚴向晶状体迁移的效果。尾蚴的最佳感染年龄为从螺体逸出后的0 - 5小时，对于经腹腔注射的双穴吸虫蚴，为体外经鱼皮转化后5小时。将鱼的整个身体或头部暴露于尾蚴后，后囊蚴在5小时首次出现在晶状体中，其数量逐渐增加直至22小时。将虹鳟的尾部区域暴露于尾蚴后，后囊蚴在14小时首次出现在晶状体中。与尾部区域相比，将鱼头暴露于尾蚴后，鱼晶状体中形成的后囊蚴明显更多；将头部或整个身体暴露于尾蚴后，40%穿透的尾蚴到达鱼的晶状体，经腹腔、肌肉或心脏注射的尾蚴或双穴吸虫蚴有20%到达晶状体，而将尾部区域暴露于尾蚴后只有5%的尾蚴形成后囊蚴。
