Stables J N, Chappell L H
Parasitology. 1986 Aug;93 ( Pt 1):71-9. doi: 10.1017/s0031182000049830.
The relationship between infection rates of rainbow trout (Salmo gairdneri) by Diplostomum spathaceum and cercarial concentration, water flow rate and temperature were investigated by means of controlled infections within a flume. A linear relationship was obtained between cercarial concentration and mean abundance of metacercariae/fish. A biphasic relationship occurred between flow rate and abundance of metacercariae. Within the confines of the flume, it was possible to control the infection rate of trout with D. spathaceum cercariae by manipulating flow rate, suggesting that it may be a possible method of controlling diplostomiasis on fish farms. No infection occurred in fish infected and maintained below 10 degrees C and the optimum infection temperature was approximately 17.5 degrees C. Infections became established in fish infected at 7.5 and 5 degrees C but maintained at 15 degrees C prior to examination. Trout were infected at 7.5 degrees C for 10-50 min and all attached cercariae were washed off and removed from the flume. Following infection, fish were either maintained at 7.5 degrees C or 15 degrees C prior to examination. Using this method, it was possible to ascertain that it was migration and not penetration which was inhibited at water temperatures below 10 degrees C.
通过在水槽内进行受控感染，研究了虹鳟（Salmo gairdneri）被匙形双腔吸虫（Diplostomum spathaceum）感染的速率与尾蚴浓度、水流速度和温度之间的关系。尾蚴浓度与平均每尾鱼的后尾蚴丰度之间呈线性关系。后尾蚴丰度与水流速度之间呈双相关系。在水槽范围内，通过操纵水流速度可以控制虹鳟被匙形双腔吸虫尾蚴的感染率，这表明这可能是一种控制养鱼场双腔吸虫病的可行方法。在10摄氏度及以下感染并饲养的鱼未发生感染，最佳感染温度约为17.5摄氏度。在7.5摄氏度和5摄氏度感染但在检查前饲养在15摄氏度的鱼发生了感染。虹鳟在7.5摄氏度下感染10 - 50分钟，然后将所有附着的尾蚴冲洗掉并从水槽中移出。感染后，在检查前将鱼饲养在7.5摄氏度或15摄氏度。使用这种方法，可以确定在水温低于10摄氏度时受到抑制的是迁移而不是穿透。
