Zhang Chun-Hui, Ma Yu-Jiu, Yang Fang-Xiao, Liu Wei, Zhang Yue-Dong
Key Laboratory of Biofuel, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Songling road 189, Qingdao 266071, China.
Bioresour Technol. 2009 Sep;100(18):4284-8. doi: 10.1016/j.biortech.2009.03.022. Epub 2009 Apr 28.
The optimal medium for butyric acid production by Clostridium thermobutyricum in a shake flask culture was studied using statistical experimental design and analysis. The optimal composition of the fermentation medium for maximum butyric acid yield, as determined on the basis of a three-level four-factor Box-Behnken design (BBD), was obtained by response surface methodology (RSM). The high correlation between the predicted and observed values indicated the validity of the model. A maximum butyric acid yield of 12.05 g/l was obtained at K(2)HPO(4) 7.2 g/l, 34.9 g/l glucose, 20 g/l yeast extract, and 15 g/l acetate, which compared well to the predicated production of 12.13 g/l.
采用统计实验设计与分析方法，研究了嗜热丁酸梭菌在摇瓶培养中生产丁酸的最佳培养基。基于三水平四因素的Box-Behnken设计（BBD）确定了最大丁酸产量的发酵培养基最佳组成，通过响应面法（RSM）获得。预测值与观测值之间的高度相关性表明该模型的有效性。在K₂HPO₄ 7.2 g/L、葡萄糖34.9 g/L、酵母提取物20 g/L和乙酸盐15 g/L的条件下，丁酸最大产量为12.05 g/L，与预测产量12.13 g/L相当。
