Guo Zhengyan, Shen Ling, Ji Zhiqin, Wu Wenjun
State Key Laboratory of Crop Stress Biology in Arid Areas, Northwest A & F University, Yangling 712100, China.
Anhui provincial laboratory of Agro-Food safety, Resources & Environment College, Anhui Agricultural University, Hefei 230036, China.
Int J Mol Sci. 2012;13(4):5230-5241. doi: 10.3390/ijms13045230. Epub 2012 Apr 24.
NW-G01, produced by Streptomyces alboflavus 313, is a novel cyclic hexapeptide antibiotic with many potential applications, including antimicrobial activity and antitumor agents. This study developed a system for optimizing medium components in order to enhance NW-G01 production. In this study, Plackett-Burman design (PBD) was used to find the key ingredients of medium components, and then response surface methodology (RSM) was implemented to determine their optimal concentrations. The results of PBD revealed that the crucial ingredients related to the production of NW-G01 were (NH(4))(2)SO(4), peptone and CaCO(3). A prediction model has been built in the experiments of central composite design and response surface methodology, and its validation has been further verified. The optimal medium composition was determined (g/L): corn starch 15, glucose 15, peptone 3.80, (NH(4))(2)SO(4) 0.06, NaCl 1.5, CaCO(3) 1.30, MgSO(4)·7H(2)O 0.015, K(2)HPO(4)·3H(2)O 0.015, MnCl(2)·4H(2)O 0.015, FeSO(4)·7H(2)O 0.015, and ZnSO(4)·7H(2)O 0.015. Compared with NW-G01 production (5.707 mg/L) in non-optimized fermentation medium, the production of NW-G01 (15.564 mg/L) in optimized fermentation medium had a 2.73-fold increase.
NW-G01由黄白链霉菌313产生，是一种新型环六肽抗生素，具有许多潜在应用，包括抗菌活性和抗肿瘤作用。本研究开发了一种优化培养基成分的系统，以提高NW-G01的产量。在本研究中，采用Plackett-Burman设计（PBD）来寻找培养基成分中的关键成分，然后实施响应面方法（RSM）来确定其最佳浓度。PBD结果表明，与NW-G01产生相关的关键成分是硫酸铵、蛋白胨和碳酸钙。在中心复合设计和响应面方法的实验中建立了预测模型，并进一步验证了其有效性。确定了最佳培养基组成（g/L）：玉米淀粉15、葡萄糖15、蛋白胨3.80、硫酸铵0.06、氯化钠1.5、碳酸钙1.30、硫酸镁·7水合物0.015、磷酸氢二钾·3水合物0.015、氯化锰·4水合物0.015、硫酸亚铁·7水合物0.015和硫酸锌·7水合物0.015。与非优化发酵培养基中NW-G01的产量（5.707 mg/L）相比，优化发酵培养基中NW-G01的产量（15.564 mg/L）提高了2.73倍。
