Lartigue Marie-Frédérique, Héry-Arnaud Geneviève, Haguenoer Eve, Domelier Anne-Sophie, Schmit Pierre-Olivier, van der Mee-Marquet Nathalie, Lanotte Philippe, Mereghetti Laurent, Kostrzewa Markus, Quentin Roland
Université François-Rabelais de Tours, UFR de Médecine, EA 3854 Bactéries et risque materno-foetal, Tours, France.
J Clin Microbiol. 2009 Jul;47(7):2284-7. doi: 10.1128/JCM.00175-09. Epub 2009 Apr 29.
Variations in proteins related to bacterial diversity may affect species identification performed using matrix-assisted laser desorption ionization (MALDI)-time of flight mass spectrometry. Using this method, we identified 110 Streptococcus agalactiae isolates characterized by serotyping and multilocus sequence typing. Serotype III and sequence type 23 strains expressed the widest variation in molecular weight of putative "species-identifying" biomarker ions. Recognition of the diversity of MALDI patterns observed in strains that represent all major intraspecies lineages assists in the constitution of an optimal reference database.
与细菌多样性相关的蛋白质变异可能会影响使用基质辅助激光解吸电离(MALDI)-飞行时间质谱进行的物种鉴定。使用这种方法,我们鉴定了110株无乳链球菌分离株,通过血清分型和多位点序列分型进行表征。血清型III和序列型23菌株在假定的“物种识别”生物标志物离子的分子量方面表现出最广泛的变异。认识到在代表所有主要种内谱系的菌株中观察到的MALDI模式的多样性有助于构建最佳参考数据库。