Subauste C S, Koniaris A H, Remington J S
Department of Immunology and Infectious Diseases, Palo Alto Medical Foundation, CA 94301.
J Immunol. 1991 Dec 1;147(11):3955-9.
Studies were performed to determine whether CTL against Toxoplasma gondii-infected cells could be induced in a murine model of T. gondii infection in which CD8+ T lymphocytes have been shown to play a major role in resistance against this parasite. In 51Cr release assays, nylon wool nonadherent spleen cells from BALB/c (H-2d) mice immunized with the temperature-sensitive (ts-4) mutant strain of T. gondii were cytotoxic for T. gondii-infected P815 (H-2d) mastocytoma cells but not for uninfected cells. This cytotoxic activity was remarkably increased after in vitro stimulation with T. gondii-infected syngeneic spleen cells. The effector cells were shown to be CD8+ T lymphocytes, because the cytotoxicity was significantly inhibited by depletion of CD8+ T lymphocytes but not by depletion of CD4+ T lymphocytes. This cytotoxic activity was genetically restricted. Spleen cells from T. gondii-immune BALB/c mice were not cytotoxic for T. gondii-infected EL4 (H-2b) thymoma cells, whereas spleen cells from T. gondii-immune C57B1/6 (H-2b) mice were cytotoxic for T. gondii-infected EL4 cells but not for T. gondii-infected P815 cells. The cytolytic activity of CD8+ T lymphocytes against T. gondii-infected cells might be a mechanism whereby these cells confer resistance against T. gondii.
在弓形虫感染的小鼠模型中开展了多项研究，以确定是否能诱导出针对弓形虫感染细胞的细胞毒性T淋巴细胞（CTL），在该模型中，已证明CD8 + T淋巴细胞在抵抗这种寄生虫方面发挥主要作用。在51Cr释放试验中，用弓形虫温度敏感（ts-4）突变株免疫的BALB/c（H-2d）小鼠的尼龙毛非黏附脾细胞，对弓形虫感染的P815（H-2d）肥大细胞瘤细胞具有细胞毒性，但对未感染的细胞无细胞毒性。在用弓形虫感染的同基因脾细胞进行体外刺激后，这种细胞毒性活性显著增强。效应细胞显示为CD8 + T淋巴细胞，因为CD8 + T淋巴细胞的耗竭可显著抑制细胞毒性，而CD4 + T淋巴细胞的耗竭则无此作用。这种细胞毒性活性受到遗传限制。弓形虫免疫的BALB/c小鼠的脾细胞对弓形虫感染的EL4（H-2b）胸腺瘤细胞无细胞毒性，而弓形虫免疫的C57B1/6（H-2b）小鼠的脾细胞对弓形虫感染的EL4细胞具有细胞毒性，但对弓形虫感染的P815细胞无细胞毒性。CD8 + T淋巴细胞对弓形虫感染细胞的溶细胞活性可能是这些细胞赋予抗弓形虫抵抗力的一种机制。
