Antigen detection in invasive aspergillosis.

The application of a reverse passive latex agglutination test and dot-blot assay are reported in the diagnosis of 50 proven cases of invasive aspergillosis and 28 suspected cases. At a latex titre cut off of greater than or equal to 1 in 8 the test had a sensitivity of 29.4%, specificity of 96.3% and efficiency of 41.4%. This was in sera taken when the diagnosis was first suggested. The sensitivity rose to 55.1% when sera with the maximum level of antigen were examined. The dot blot was more sensitive with 33.3% of cases being positive in the initial sera. This increased to 61.5% when the serum with the maximum antigen level was taken.