Kner Peter, Chhun Bryant B, Griffis Eric R, Winoto Lukman, Gustafsson Mats G L
Department of Biochemistry and Biophysics, San Francisco, California, USA.
Nat Methods. 2009 May;6(5):339-42. doi: 10.1038/nmeth.1324. Epub 2009 Apr 26.
Structured-illumination microscopy can double the resolution of the widefield fluorescence microscope but has previously been too slow for dynamic live imaging. Here we demonstrate a high-speed structured-illumination microscope that is capable of 100-nm resolution at frame rates up to 11 Hz for several hundred time points. We demonstrate the microscope by video imaging of tubulin and kinesin dynamics in living Drosophila melanogaster S2 cells in the total internal reflection mode.
结构光照明显微镜可将宽场荧光显微镜的分辨率提高一倍，但此前对于动态实时成像来说速度过慢。在此，我们展示了一种高速结构光照明显微镜，它在高达11赫兹的帧率下能够实现100纳米的分辨率，可进行数百个时间点的成像。我们通过在全内反射模式下对活的黑腹果蝇S2细胞中的微管蛋白和驱动蛋白动力学进行视频成像，展示了该显微镜。
