Department of Immunopathology, Iuliu Hatieganu University of Medicine and Pharmacy, Str Croitorilor no 19-21, 3400 Cluj-Napoca, Romania.
Rheumatol Int. 2009 Dec;30(2):181-5. doi: 10.1007/s00296-009-0933-4.
An increased level of apoptotic material and B cell activation leading to autoantibody production are hallmarks of systemic lupus erythematoses (SLE). Increased FAS expression, apoptosis, and CD154-mediated signaling, enabling T–B cell interaction are involved in the pathogenesis of SLE. This study addresses the expression profile of CD154 and FAS in the peripheral blood of patients with SLE, rheumatoid arthritis (RA) and normal healthy control donors. Surface markers on peripheral blood T and B cells from patients and healthy control donors were assessed using flow cytometry. The expression of CD154 and FAS were significantly increased in T and B cells of SLE patients as compared to healthy control donors and RA patients. In SLE and RA patients, FAS expression strongly correlated with CD154 expression on T cells, which was not found in healthy control donors. FAS expression was also associated with the occurrence of anti-DNA antibodies. We demonstrate high CD154 and FAS expression as a characteristic feature of SLE. This pattern may reflect simultaneous activation of apoptosis and activation of B–T cell interaction in SLE.
凋亡物质水平升高和 B 细胞激活导致自身抗体产生是红斑狼疮（SLE）的特征。FAS 表达增加、凋亡和 CD154 介导的信号转导，使 T-B 细胞相互作用参与了 SLE 的发病机制。本研究探讨了 CD154 和 FAS 在 SLE、类风湿关节炎（RA）患者和正常健康对照供者外周血中的表达谱。使用流式细胞术评估患者和健康对照供者外周血 T 和 B 细胞表面标志物。与健康对照供者和 RA 患者相比，SLE 患者的 T 和 B 细胞中 CD154 和 FAS 的表达显著增加。在 SLE 和 RA 患者中，FAS 表达与 T 细胞上的 CD154 表达强烈相关，而在健康对照供者中则未发现这种相关性。FAS 表达也与抗 DNA 抗体的发生有关。我们证明了 CD154 和 FAS 的高表达是 SLE 的特征。这种模式可能反映了 SLE 中同时发生的凋亡激活和 B-T 细胞相互作用的激活。
