Akinrinmade Fadeyemi J, Akinrinde Akinleye S, Soyemi Olubisi O, Oyagbemi Ademola A
a Department of Veterinary Surgery and Reproduction, Faculty of Veterinary Medicine, University of Ibadan , Ibadan , Nigeria.
b Department of Veterinary Physiology, Biochemistry and Pharmacology, Faculty of Veterinary Medicine, University of Ibadan , Ibadan , Nigeria.
J Diet Suppl. 2016;13(4):420-32. doi: 10.3109/19390211.2015.1103828. Epub 2015 Dec 3.
Parquetina nigrescens is a medicinal herb with recognized antioxidant properties and potential to alleviate conditions associated with oxidative stress, including gastric ulcers. We investigated the protective potential of methanol extract of Parquetina nigrescens (MEPN) against ischemia-reperfusion injury in the intestine of rats. Thirty (30) male Wistar albino rats were randomly assigned into five groups with Group I made up of control rats and Group II consisting of rats experimentally subjected to ischemia and reperfusion (IR) by clamping of the superior mesenteric artery (SMA) for 30 minutes and 45 minutes, respectively. Groups III and IV rats also had IR, but were initially pre-treated with MEPN at 500 mg/kg and 1000 mg/kg respectively, for seven days. Rats in Group V were also pre-treated with Vitamin C, for seven days, before induction of IR. The results showed marked reduction in intestinal epithelial lesions in groups treated with MEPN, compared to the IR group which had severe villi erosion, inflammatory cell infiltration and hemorrhages. There were significant increases in Malondialdehyde (MDA) and significant reductions in reduced glutathione (GSH) and Glutathione S-transferase (GST) activity with IR injury, while pre-treatment with either MEPN or Vitamin C prevented these effects. Increases in Glutathione peroxidase (GPX), Catalase (CAT) and Superoxide dismutase (SOD) with IR provided evidence for adaptive responses to oxidative injury during IR and preservation of enzyme activity by MEPN and Vitamin C. Taken together, Parquetina nigrescens provided considerable alleviation of intestinal injury produced by IR, at values much as effective as that offered by Vitamin C.
黑拟耳草是一种具有公认抗氧化特性的草药，有潜力缓解与氧化应激相关的病症，包括胃溃疡。我们研究了黑拟耳草甲醇提取物（MEPN）对大鼠肠道缺血再灌注损伤的保护潜力。30只雄性Wistar白化大鼠被随机分为五组，第一组为对照大鼠，第二组由通过夹闭肠系膜上动脉（SMA）分别进行30分钟和45分钟缺血再灌注（IR）实验的大鼠组成。第三组和第四组大鼠也进行了IR，但最初分别用500mg/kg和1000mg/kg的MEPN预处理7天。第五组大鼠在诱导IR前也用维生素C预处理7天。结果显示，与有严重绒毛侵蚀、炎性细胞浸润和出血的IR组相比，用MEPN处理的组肠道上皮损伤明显减轻。IR损伤导致丙二醛（MDA）显著增加，还原型谷胱甘肽（GSH）和谷胱甘肽S-转移酶（GST）活性显著降低，而用MEPN或维生素C预处理可防止这些影响。IR时谷胱甘肽过氧化物酶（GPX）、过氧化氢酶（CAT）和超氧化物歧化酶（SOD）增加，为IR期间对氧化损伤的适应性反应以及MEPN和维生素C对酶活性的保存提供了证据。综上所述，黑拟耳草对IR所致的肠道损伤有相当程度的缓解作用，其效果与维生素C相当。
