Preethi Korengath Chandran, Kuttan Ramadasan
Department of Biochemistry Amala Cancer Research Centre, Amala Nagar, Thrissur, India, 680 555.
Indian J Exp Biol. 2009 Mar;47(3):163-8.
Flower extract of C. officinalis L. was evaluated for its protective effect against CCl4 induced acute hepatotoxicity and cisplatin induced nephrotoxicity. The activities of serum marker enzymes of liver injury like glutamate pyruvate transaminase (SGPT), glutamate oxaloacetate transaminase (SGOT) and alkaline phosphatase (ALP) which were increased by CCl4 injection was found to be significantly reduced by the pretreatment of the flower extract at 100 and 250 mg/kg body weight. The lipid peroxidation in liver, the marker of membrane damage and the total bilirubin content in serum were also found to be at significantly low level in the extract pretreated group, indicating its protective role. The kidney function markers like urea and creatinine were significantly increased in cisplatin treated animals. However, their levels were found to be lowered in the extract pretreated groups (100 and 250 mg/kg body weight). Moreover, cisplatin induced myelosuppression was ameliorated by the extract pretreatment. Treatment with the extract produced enhancement of antioxidant enzymes--superoxide dismutase and catalase and glutathione. Results suggest a protective role of the flower extract of C. officinalis against CCl4 induced acute hepatotoxicity and cisplatin induced nephrotoxicity. Extract has been found to contain several carotenoids of which lutein, zeaxanthin and lycopene predominates. Possible mechanism of action of the flower extract may be due to its antioxidant activity and reduction of oxygen radicals.
对药用金盏花的花提取物进行了评估，以研究其对四氯化碳诱导的急性肝毒性和顺铂诱导的肾毒性的保护作用。通过注射四氯化碳而升高的肝损伤血清标志物酶，如谷丙转氨酶（SGPT）、谷草转氨酶（SGOT）和碱性磷酸酶（ALP）的活性，在以100和250mg/kg体重的剂量进行花提取物预处理后，发现显著降低。在提取物预处理组中，还发现肝脏中的脂质过氧化（膜损伤的标志物）和血清中的总胆红素含量处于显著较低水平，表明其保护作用。在顺铂处理的动物中，肾功能标志物如尿素和肌酐显著升高。然而，在提取物预处理组（100和250mg/kg体重）中，它们的水平有所降低。此外，提取物预处理改善了顺铂诱导的骨髓抑制。提取物处理使抗氧化酶——超氧化物歧化酶、过氧化氢酶和谷胱甘肽增强。结果表明，药用金盏花花提取物对四氯化碳诱导的急性肝毒性和顺铂诱导的肾毒性具有保护作用。已发现提取物含有几种类胡萝卜素，其中叶黄素、玉米黄质和番茄红素占主导。花提取物可能的作用机制可能是由于其抗氧化活性和减少氧自由基。
