Verma Pawan Kumar, Raina Rajinder, Sultana Mudasir, Singh Maninder, Kumar Pawan
a Division of Veterinary Pharmacology & Toxicology , Division of Pharmacology, FVSC & AH, Sher-e-Kashmir University of Agricultural Sciences & Technology - Jammu , RS Pura, Jammu, Jammu & Kashmir , India .
b Division of Veterinary Pharmacology and Toxicology , Faculty of Veterinary Sciences and Animal Husbandry , RS Pura, Jammu, Jammu & Kashmir , India .
Ren Fail. 2016;38(1):142-50. doi: 10.3109/0886022X.2015.1103585. Epub 2015 Oct 29.
The present study was aimed to determine the total antioxidant status (TAS), total oxidant status (TOS) and oxidative stress index (OSI) of plasma and renal tissue in cisplatin (cDDP) induced nephrotoxic rats and its protection by treatments with floral extracts of Calendula officinalis Linn. Treatment with cDDP elevated (p < 0.05) the levels of blood urea nitrogen, creatinine (CR), TOS, OSI and malondialdehyde (MDA) but lowered (p < 0.05) total plasma proteins, TAS, total thiols (TTH), blood glutathione (GSH) and antioxidant enzymes compared to the control group. Pre- and post-treatments of ethanolic floral extract of C. officinalis along with cDDP restored (p > 0.05) CR, albumin, TOS, GSH and activities of antioxidant enzymes in blood and renal tissue. Ethanolic extract treatments reduced (p < 0.05) MDA level in renal tissue without restoring the erythrocyte MDA level following cDDP treatment. These observations were further supported by the histopathological findings in renal tissue. Observations of the present study have shown that treatments with ethanolic floral extract of C. officinalis protect cDDP induced nephrotoxicity by restoring antioxidant system of the renal tissue.
本研究旨在测定顺铂（cDDP）诱导的肾毒性大鼠血浆和肾组织中的总抗氧化状态（TAS）、总氧化状态（TOS）和氧化应激指数（OSI），以及金盏花（Calendula officinalis Linn.）花提取物处理对其的保护作用。与对照组相比，cDDP处理使血尿素氮、肌酐（CR）、TOS、OSI和丙二醛（MDA）水平升高（p < 0.05），但使血浆总蛋白、TAS、总巯基（TTH）、血谷胱甘肽（GSH）和抗氧化酶水平降低（p < 0.05）。金盏花花乙醇提取物与cDDP联合进行预处理和后处理，可使血液和肾组织中的CR、白蛋白、TOS、GSH及抗氧化酶活性恢复（p > 0.05）。乙醇提取物处理可降低肾组织中的MDA水平（p < 0.05），但在cDDP处理后未能恢复红细胞MDA水平。肾组织的组织病理学结果进一步支持了这些观察结果。本研究的观察结果表明，金盏花花乙醇提取物处理通过恢复肾组织的抗氧化系统来保护cDDP诱导的肾毒性。
