Department of Clinical Science, Division of Obstetrics and Gynaecology, Karolinska Institutet at Karolinska University Hospital Huddinge, Stockholm, Sweden.
Fertil Steril. 2010 Jul;94(2):701-7. doi: 10.1016/j.fertnstert.2009.03.042. Epub 2009 May 5.
To investigate whether idiopathic menorrhagia (IM) is associated with alterations of the vascular expression of angiopoietin-1, angiopoietin-2, and tie-2 receptor.
Prospective clinical study.
University Hospital, Department of Gynecology.
PATIENT(S): Twenty-four patients with IM and 18 women with eumenorrhea.
INTERVENTION(S): Endometrial samples underwent immunohistochemical staining for CD34, angiopoetin-1, angiopoietin-2, tie-2, and smooth muscle actin-alpha. Previously published data on gap size and expression of vascular endothelial growth factor family members were used.
MAIN OUTCOME MEASURE(S): Differences in immunostaining for these markers by computer-assisted stereological analysis.
RESULT(S): There was significantly more angiopoetin-1 positive vessels in IM in the secretory phase, but not of angiopoetin-2 and tie-2, compared with controls. Densities of angiopoetin-1 positive vessels correlated significantly to those of angiopoetin-2 and vascular endothelial growth factor receptor 3. Smooth muscle actin-alpha positive pericytes covered the gaps. Double staining for CD34 and tie-2 receptor was partly identical, but gaps were covered by tie-2 stain.
CONCLUSION(S): The discrete deregulation observed of the angiopoetin-1 expression before menstruation might affect vascular integrity, thereby contributing to the excessive blood loss in IM.
研究特发性月经过多（IM）是否与血管生成素-1、血管生成素-2 和 tie-2 受体的血管表达改变有关。
前瞻性临床研究。
大学医院妇科。
24 例 IM 患者和 18 例月经正常的妇女。
子宫内膜标本行 CD34、血管生成素-1、血管生成素-2、tie-2 和平滑肌肌动蛋白-α免疫组织化学染色。使用了先前发表的关于血管内皮生长因子家族成员的间隙大小和表达的数据。
通过计算机辅助立体学分析这些标记物的免疫染色差异。
与对照组相比，在分泌期，IM 中血管生成素-1 阳性血管明显更多，但血管生成素-2 和 tie-2 则不然。血管生成素-1 阳性血管的密度与血管生成素-2 和血管内皮生长因子受体 3 的密度显著相关。平滑肌肌动蛋白-α阳性周细胞覆盖了间隙。CD34 和 tie-2 受体的双重染色部分相同，但间隙被 tie-2 染色覆盖。
在月经前观察到的血管生成素-1 表达的离散失调可能会影响血管完整性，从而导致 IM 中过度出血。
