Lab. for Molecular Cell Biology, Faculty of Medicine and IBMC, Univ. of Porto, Al. Prof. Hernâni Monteiro, 4200-319 Porto, Portugal.
J Food Sci. 2010 Apr;75(3):H79-86. doi: 10.1111/j.1750-3841.2010.01527.x.
Long-term consumption of red wine (RW) apparently confers some protection against cardiovascular diseases due to antiatherosclerotic properties of polyphenols and ethanol (EtOH). There is some evidence indicating that they do so by regulating angiogenesis, but the mechanism and the modulator factors involved are largely unknown. The aim of this study was to evaluate the effects of chronic ingestion of RW in vascular structure and in the pattern of expression of vascular growth factors in the rat corpus cavernosum. Male Wistar rats aged 6 mo were treated with RW or an equivalent EtOH solution, as the only liquid source for 6 mo. Expression of vascular endothelial growth factor (VEGF), angiopoietin 1 and angiopoietin 2, and their receptors (VEGFR1, VEGFR2, and Tie2) in cavernous tissue was assayed by immunofluorescence and Western blotting. A reduction of VEGF and VEGFR2 expression, respectively, in smooth muscle and endothelial cells was observed in RW-treated animals, which was balanced by an increase in angiopoietins/Tie2 expression. In EtOH rats, only a decrease in expression of the receptors VEGFR2 and Tie2 was observed. These results, taken together, suggest that antioxidants present in RW activate selected mechanisms for the maintenance of cavernous tissue vascularization. However, functional studies will be necessary to elucidate if RW is of benefit in the prevention of deleterious vascular events associated with ED.
长期饮用红酒（RW）显然由于多酚和乙醇（EtOH）的抗动脉粥样硬化特性对心血管疾病有一定的保护作用。有一些证据表明，它们通过调节血管生成来实现这一点，但涉及的机制和调节剂因素在很大程度上尚不清楚。本研究旨在评估慢性摄入 RW 对大鼠海绵体血管结构和血管生长因子表达模式的影响。6 月龄雄性 Wistar 大鼠用 RW 或等量的 EtOH 溶液处理，作为唯一的液体来源 6 个月。通过免疫荧光和 Western blot 检测海绵组织中血管内皮生长因子（VEGF）、血管生成素 1 和血管生成素 2 及其受体（VEGFR1、VEGFR2 和 Tie2）的表达。在 RW 处理的动物中，观察到平滑肌和内皮细胞中 VEGF 和 VEGFR2 表达分别减少，这被血管生成素/Tie2 表达的增加所平衡。在 EtOH 大鼠中，仅观察到受体 VEGFR2 和 Tie2 的表达下降。这些结果表明，RW 中存在的抗氧化剂激活了维持海绵体组织血管生成的特定机制。然而，需要进行功能研究以阐明 RW 是否有益于预防与 ED 相关的有害血管事件。
