Department of Chromatography, Tallinn Laboratory, Estonian Veterinary and Food Laboratory, 11415 Tallinn, Estonia.
J Chromatogr A. 2009 Nov 13;1216(46):8080-9. doi: 10.1016/j.chroma.2009.04.005. Epub 2009 Apr 9.
Thyreostatic compounds could be illegally administered to animals in order to obtain a weight gain due to a higher retention of water in the edible tissue and the gastro-intestinal tract. In the European Union their use for animal production is banned since 1981. Recently a highly sensitive method exploiting the determination of thyreostats with 3-iodobenzylbromide prior to purification to determine thyreostats in urine and other matrices was reported. For the first time, the UPLC instrumentation was used to separate the 3-iodobenzyl derivatives of various thyreostats. The deuterated internal standards tapazole-d(3) and propylthiouracil-d(5) were for the first time used for the quantification of tapazole, thiouracil, methylthiouracil, propylthiouracil, phenylthiouracil and mercaptobenzimidazole. The confirmative quantitative liquid chromatographic tandem mass spectrometric (LC-MS/MS) method was validated according to Commission Decision 2002/657/EC. The decision limit (CCalpha) and the detection capability (CCbeta) were found to be for all compounds below the recommended value of 10 microg kg(-1).
为了使食用组织和胃肠道保留更多水分而使动物体重增加，可能会将甲状腺抑制剂非法施用于动物。自 1981 年以来，欧盟已禁止将其用于动物生产。最近，人们报道了一种非常灵敏的方法，该方法利用 3-碘苄基溴化物在纯化前测定甲状腺抑制剂，从而在尿液和其他基质中测定甲状腺抑制剂。首次使用 UPLC 仪器分离各种甲状腺抑制剂的 3-碘苄基衍生物。氘代内标物他唑巴坦-d（3）和丙基硫氧嘧啶-d（5）首次用于他唑巴坦、硫脲嘧啶、甲硫氧嘧啶、丙基硫氧嘧啶、苯硫氧嘧啶和巯基苯并咪唑的定量。根据委员会第 2002/657/EC 号决定对确认定量液相色谱串联质谱（LC-MS/MS）方法进行了验证。发现所有化合物的决策限值（CCalpha）和检测能力（CCbeta）均低于建议的 10μgkg（-1）值。
