Chen Ying-Chieh, Lee Don-Ching, Hsiao Chao-Yang, Chung Yu-Fen, Chen Huang-Chin, Thomas Joseph P, Pong Way-Faung, Tai Nyan-Hwa, Lin I-Nan, Chiu Ing-Ming
Department of Materials Science and Engineering, National Tsing-Hua University, Hsin-Chu, Taiwan.
Biomaterials. 2009 Jul;30(20):3428-35. doi: 10.1016/j.biomaterials.2009.03.058. Epub 2009 Apr 29.
The interaction of ultra-nanocrystalline diamond (UNCD) with neural stem cells (NSCs) has been studied along with its surface modification in order to improve its function as a biomaterial. Hydrogen- and oxygen-terminated UNCD films were compared with standard grade polystyrene in terms of their impact on the growth, expansion and differentiation of NSCs. When NSCs were cultured on these substrates in low serum and without any differentiating factors, hydrogen-terminated UNCD films spontaneously induced cell proliferation and neuronal differentiation. Oxygen-terminated UNCD films were also shown to further improve neural differentiation, with a preference to differentiate into oligodendrocytes. Hence, controlling the surface properties of UNCD could manipulate the differentiation of NSCs for different biomedical applications. These observations raise the potential for the use of UNCD as a biomaterial for central nervous system transplantation and tissue engineering.
为了改善其作为生物材料的功能，人们研究了超纳米晶金刚石（UNCD）与神经干细胞（NSCs）的相互作用及其表面改性。将氢端化和氧端化的UNCD薄膜与标准级聚苯乙烯在对NSCs的生长、扩增和分化的影响方面进行了比较。当NSCs在低血清且无任何分化因子的情况下在这些基质上培养时，氢端化的UNCD薄膜能自发诱导细胞增殖和神经元分化。氧端化的UNCD薄膜也被证明能进一步促进神经分化，且更倾向于分化为少突胶质细胞。因此，控制UNCD的表面性质可以调控NSCs的分化，以用于不同的生物医学应用。这些观察结果提高了将UNCD用作中枢神经系统移植和组织工程生物材料的可能性。
