Departament de Fisiologia (Farmàcia) and Institut de Recerca en Nutrició i Seguretat Alimentària (INSA-UB), Universitat de Barcelona, Spain.
J Pharm Biomed Anal. 2010 Jan 20;51(2):391-8. doi: 10.1016/j.jpba.2009.03.026. Epub 2009 Apr 5.
trans-Resveratrol, a polyphenol from grapes, is being recognized as a bioactive agent with potential beneficial effects on health. However, little is known about its distribution in the organism mainly because of the lack of accurate and precise detection methods. Consequently the aim of the present study was to develop a methodology of extraction and quantification of trans-resveratrol and its metabolites in plasma, brain, testis, liver, lungs and kidney by HPLC. To this end, the time of homogenization and liquid extraction were adapted to the different tissues. The methods were validated using homogenized tissues spiked with pure trans-resveratrol. The precision (% R.S.D.) ranged from 3.7% in testis to 13.2% in lungs. Recoveries were 98.5+/-3.2% (liver), 100.1+/-1.8% (kidney), 96.5+/-7.6% (lungs), 99.0+/-0.7% (brain) and 103+/-2.7% (testicle). The limits of detection ranged from 5.5 nM in testis to 11.2 nM in kidney. After validation, the methods were applied to the assessment of the bioavailability and distribution of trans-resveratrol in rats after the intravenous administration of 15 mg/kg. At 90 min, trans-resveratrol and its glucuronide and sulfate conjugates were widely distributed in all the tissues studied. The highest concentrations (nmol/g tissue) were found in kidney (resveratrol: 1.45+/-0.35; glucuronide: 2.91+/-0.19; sulfate: not detected), and the lowest in brain (resveratrol: 0.17+/-0.04; glucuronide: not detected; sulfate: 0.04+/-0.01). In conclusion, accurate and reproducible methods have been described to identify target tissues of resveratrol as a first step to understand its mechanisms of action in vivo.
反式白藜芦醇是一种来源于葡萄的多酚，它被认为是一种具有潜在健康益处的生物活性物质。然而，由于缺乏准确和精确的检测方法，其在体内的分布情况知之甚少。因此，本研究旨在建立一种高效液相色谱法提取和定量检测血浆、脑、睾丸、肝、肺和肾中反式白藜芦醇及其代谢物的方法。为此，适应不同组织匀浆和液体提取的时间。使用纯反式白藜芦醇对组织匀浆进行加标，验证方法的精密度（%RSD）范围为 3.7%（睾丸）至 13.2%（肺）。回收率为 98.5+/-3.2%（肝）、100.1+/-1.8%（肾）、96.5+/-7.6%（肺）、99.0+/-0.7%（脑）和 103+/-2.7%（睾丸）。检测限范围为 5.5 nM（睾丸）至 11.2 nM（肾）。验证后，该方法应用于评估大鼠静脉注射 15 mg/kg 后反式白藜芦醇的生物利用度和分布。90 分钟时，反式白藜芦醇及其葡萄糖醛酸和硫酸盐缀合物广泛分布于所有研究组织中。组织中浓度最高（nmol/g 组织）的为肾（白藜芦醇：1.45+/-0.35；葡萄糖醛酸：2.91+/-0.19；硫酸盐：未检出），脑浓度最低（白藜芦醇：0.17+/-0.04；葡萄糖醛酸：未检出；硫酸盐：0.04+/-0.01）。总之，本研究建立了一种准确、重现性好的方法来鉴定白藜芦醇的靶组织，作为理解其体内作用机制的第一步。
