Departament de Fisiologia (Farmàcia) and Institut de Recerca en Nutrició i Seguretat Alimentària (INSA-UB), Universitat de Barcelona, Spain.
J Agric Food Chem. 2010 Jul 14;58(13):8104-10. doi: 10.1021/jf100702x.
trans-Resveratrol, a natural occurring polyphenol, has been described as an antiproliferative and proapoptotic agent in vitro. Here, we studied the effect of trans-resveratrol administered orally at a dose of 60 mg/kg for 49 days on early preneoplastic markers induced by the intraperitoneal injection of 1,2-dimethylhydrazine (20 mg/kg). We measured trans-resveratrol and its derivates by liquid-liquid extraction followed by high-performance liquid chromatography diode array detection analysis in colon contents. Dihydroresveratrol was the most abundant compound in the colon, followed by trans-resveratrol glucuronide and small amounts of trans-resveratrol and its sulfate. The administration of trans-resveratrol decreased aberrant crypt foci by 52%, and mucin depleted foci by 45% in colon. In conclusion, the correlation between the reduction of precancerous colonic lesions and the availability of trans-resveratrol in the colon provides a new insight into the therapeutical potential of this polyphenol and its metabolites.
反式白藜芦醇是一种天然存在的多酚类物质，已被描述为体外具有抗增殖和促凋亡作用的物质。在这里，我们研究了腹腔注射 1,2-二甲基肼（20mg/kg）诱导早期癌前标志物后，每天口服 60mg/kg 反式白藜芦醇 49 天对其的影响。我们通过液-液萃取，然后用高效液相色谱二极管阵列检测分析结肠内容物来测量反式白藜芦醇及其衍生物。二氢白藜芦醇是结肠中含量最丰富的化合物，其次是反式白藜芦醇葡萄糖苷和少量的反式白藜芦醇及其硫酸盐。反式白藜芦醇的给药使结肠中异常隐窝病灶减少了 52%，粘蛋白耗竭病灶减少了 45%。总之，结肠中癌前病变减少与反式白藜芦醇的可用性之间的相关性为这种多酚及其代谢物的治疗潜力提供了新的见解。
