Tian Xin-Peng, Tang Shu-Kun, Dong Jun-De, Zhang Yu-Qin, Xu Li-Hua, Zhang Si, Li Wen-Jun
The Key Laboratory for Microbial Resources of the Ministry of Education, P. R. China.
Int J Syst Evol Microbiol. 2009 May;59(Pt 5):948-52. doi: 10.1099/ijs.0.005231-0.
A novel marine actinomycete, designated SCSIO 00652(T), was isolated from a marine sediment collected from the northern South China Sea at a depth of 3865 m. The strain formed branched substrate mycelia and no fragmentation was found. Abundant aerial mycelia differentiated into long spore chains and the spores had a wrinkled surface. Growth occurred on ISP medium 2 with 0-5 % (w/v) NaCl and at 10-55 degrees C. The whole-cell hydrolysate contained meso-diaminopimelic acid and glucose as the whole-cell sugar. blast search results based on an almost-complete 16S rRNA gene sequence showed the novel strain had the highest similarity (96.5 %) with Nocardiopsis trehalosi VKM Ac-942(T). The phylogenetic tree of the family Nocardiopsaceae indicated that strain SCSIO 00652(T) formed a distinct lineage at the deepest branch with a high bootstrap value. Additionally, the profiles of menaquinones, phospholipids and fatty acids showed there were marked differences between strain SCSIO 00652(T) and the recognized genera of the family Nocardiopsaceae. Based on the polyphasic data, a new genus, Marinactinospora gen. nov., is proposed within the family Nocardiopsaceae with the type species Marinactinospora thermotolerans sp. nov. The type strain of the type species is SCSIO 00652(T) (=DSM 45154(T)=CCTCC AA 208041(T)).
从中国南海北部3865米深处采集的海洋沉积物中分离出一株新型海洋放线菌，命名为SCSIO 00652(T)。该菌株形成分支状基内菌丝，未发现断裂现象。丰富的气生菌丝分化成长孢子链，孢子表面有皱纹。在含0-5%（w/v）氯化钠的ISP培养基2上，于10-55℃可生长。全细胞水解产物含有内消旋二氨基庚二酸和葡萄糖作为全细胞糖。基于近乎完整的16S rRNA基因序列的blast搜索结果显示，该新菌株与海藻糖诺卡氏菌VKM Ac-942(T)的相似度最高（96.5%）。诺卡氏菌科的系统发育树表明，菌株SCSIO 00652(T)在最深分支处形成了一个具有高自展值的独特分支。此外，甲基萘醌、磷脂和脂肪酸的图谱显示，菌株SCSIO 00652(T)与诺卡氏菌科已确认的属之间存在明显差异。基于多相数据，在诺卡氏菌科内提出一个新属，即嗜热海洋放线孢菌属（Marinactinospora gen. nov.），其模式种为嗜热海洋放线孢菌（Marinactinospora thermotolerans sp. nov.）。模式种的模式菌株为SCSIO 00652(T)（=DSM 45154(T)=CCTCC AA 208041(T)）。
