Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education, Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, People's Republic of China.
Antonie Van Leeuwenhoek. 2012 Jun;102(1):177-85. doi: 10.1007/s10482-012-9725-z. Epub 2012 Mar 28.
Two novel Gram-positive, spore-forming, thermophilic actinomycetes, designated as strain YIM 77501(T) and YIM 77570, were isolated from a sandy soil sample collected at Tengchong National Volcanic Geological Park, Yunnan province, south-west China. Phylogenetic analysis based on the 16S rRNA gene sequences suggested that the two isolates fell within the family Streptosporangiaceae. The strains formed extensively branched substrate and aerial mycelia which carried masses of long, straight or irregular spore chains composed of warty ornamented spores. Cell walls of the two strains contained meso-diaminopimelic acid and glucose, galactose, mannose and ribose were detected as whole-cell sugars. The predominant menaquinones were MK-9(H(4)) and MK-9(H(6)). The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, N-acetylglucosamine-containing phospholipids and phosphatidylinositol, phosphatidylinositolmannosides. The major cellular fatty acids were iso-C(16:0) and 10-methyl C(17:0). The DNA G+C content was 74-76 mol%. On the basis of the morphological and chemotaxonomic characteristics as well as the phylogenetic analysis, these strains represents a novel species of a new genus within the family Streptosporangiaceae, for which the name Thermoactinospora rubra gen. nov., sp. nov. is proposed. The type strain of T. rubra is YIM 77501(T) (=DSM 45614(T) = CCTCC AA 2011014(T)).
两株新型革兰阳性、产芽孢、嗜热放线菌，分别命名为菌株 YIM 77501(T) 和 YIM 77570，从中国西南部云南省腾冲国家火山地质公园的沙土样本中分离得到。基于 16S rRNA 基因序列的系统发育分析表明，这两株菌属于链霉菌科。这两株菌形成广泛分支的基质和气生菌丝，其上带有大量长而直或不规则的孢子链，由疣状装饰孢子组成。两株菌的细胞壁均含有 meso-二氨基庚二酸，且全细胞糖中检测到葡萄糖、半乳糖、甘露糖和核糖。主要的类异戊二烯醌为 MK-9(H(4)) 和 MK-9(H(6))。主要的极性脂为双磷脂酰甘油、磷脂酰乙醇胺、含有 N-乙酰葡萄糖胺的磷脂和磷脂酰肌醇、磷脂酰肌醇甘露糖苷。主要的细胞脂肪酸为 iso-C(16:0) 和 10-甲基 C(17:0)。DNA G+C 含量为 74-76 mol%。基于形态学、化学分类学特征和系统发育分析，这些菌株代表链霉菌科一个新属中的一个新种，命名为红热放线菌属 Thermoactinospora rubra 属，种名 nov.。T. rubra 的模式菌株为 YIM 77501(T) (=DSM 45614(T)=CCTCC AA 2011014(T))。
