Ehrmann Matthias A, Freiding Simone, Vogel Rudi F
Lehrstuhl für Technische Mikrobiologie, Technische Universität München, Weihenstephaner Steig 16, 85350 Freising, Germany.
Int J Syst Evol Microbiol. 2009 May;59(Pt 5):943-7. doi: 10.1099/ijs.0.005983-0.
A novel lactic acid bacterium, strain TMW 2.694(T), was isolated among other lactic acid bacteria from palm wine, an alcoholic beverage produced from the sap of various palm tree species. Strain TMW 2.694(T) is a Gram-positive, facultatively anaerobic, catalase-negative, non-spore-forming coccus, occurring in long chains. Phylogenetic analysis based on 16S rRNA gene sequencing positioned strain TMW 2.694(T) in a distinct line of descent within the genus Leuconostoc, with the closest neighbours being Leuconostoc lactis JCM 6123(T) (98.7 % sequence similarity) and Leuconostoc citreum DSM 5577(T) (98.8 % sequence similarity). Comparative sequencing of the additional phylogenetic markers dnaK and recA confirmed the 16S rRNA gene tree topology. Genomic DNA-DNA similarities of strain TMW 2.694(T) to L. lactis DSM 20202(T) and L. citreum DSM 5577(T) were 45.1 and 17.7 %, respectively. The DNA G+C content is 36.4 mol%. Thus, we propose a novel species within the genus Leuconostoc, with the name Leuconostoc palmae sp. nov. and the type strain TMW 2.694(T) (=DSM 21144(T) =LMG 24510(T)).
从棕榈酒（一种由多种棕榈树汁液制成的酒精饮料）中分离出了一种新型乳酸菌TMW 2.694(T)，它与其他乳酸菌一同被分离出来。TMW 2.694(T)菌株是革兰氏阳性、兼性厌氧、过氧化氢酶阴性、不产芽孢的球菌，呈长链状排列。基于16S rRNA基因测序的系统发育分析将TMW 2.694(T)菌株定位在明串珠菌属内一个独特的谱系中，其最接近的亲缘种是乳酸明串珠菌JCM 6123(T)（序列相似性为98.7%）和柠檬明串珠菌DSM 5577(T)（序列相似性为98.8%）。对另外的系统发育标记dnaK和recA进行比较测序，证实了16S rRNA基因树的拓扑结构。TMW 2.694(T)菌株与乳酸明串珠菌DSM 20202(T)和柠檬明串珠菌DSM 5577(T)的基因组DNA-DNA相似性分别为45.1%和17.7%。DNA的G+C含量为36.4 mol%。因此，我们提议在明串珠菌属内设立一个新种，命名为棕榈明串珠菌（Leuconostoc palmae sp. nov.），模式菌株为TMW 2.694(T)（=DSM 21144(T) =LMG 24510(T)）。
