Department of Oncology, the First Affiliated Hospital of Anhui Medical University, 218 Ji-Xi Road, Hefei, China.
Lung Cancer. 2010 Feb;67(2):227-31. doi: 10.1016/j.lungcan.2009.03.029. Epub 2009 May 5.
Due to state-of-art analytical techniques, non-invasive exhaled volatile organic compounds (VOCs) analysis has become a potential method for early diagnosis of lung cancer. We collected breath samples from 43 patients with non-small cell lung cancer (NSCLC) and 41 normal controls using Tedlar gas bags. The VOCs were extracted with solid phase micro-extraction (SPME) and analyzed by gas chromatography (GC)/mass spectrometry (MS). The number of VOCs detected in each breath sample ranged from 68 to 114. Among the VOCs 1-butanol and 3-hydroxy-2-butanone were found at significantly higher concentrations in breath of the lung cancer patients compared to the controls. VOCs levels were not significantly different between early stage lung cancer patients and late stage lung cancer patients. Lung adenocarcinoma was significantly related to higher VOCs concentrations in the breath. Our data showed that 1-butanol and 3-hydroxy-2-butanone in breath could possibly be taken as useful breath biomarkers for discerning potential lung cancer patients and VOCs analysis could be used as a complementary test for the diagnosis of lung cancer.
由于先进的分析技术，非侵入性呼出气挥发性有机化合物（VOCs）分析已成为早期诊断肺癌的一种潜在方法。我们使用 Tedlar 气袋从 43 名非小细胞肺癌（NSCLC）患者和 41 名正常对照中收集了呼气样本。使用固相微萃取（SPME）提取 VOCs，并通过气相色谱（GC）/质谱（MS）进行分析。每个呼气样本中检测到的 VOCs 数量从 68 到 114 不等。在 VOCs 中，1-丁醇和 3-羟基-2-丁酮在肺癌患者的呼气中明显高于对照组。早期和晚期肺癌患者的 VOCs 水平无显著差异。肺腺癌与呼气中较高的 VOCs 浓度显著相关。我们的数据表明，呼气中的 1-丁醇和 3-羟基-2-丁酮可能可作为鉴别潜在肺癌患者的有用呼气生物标志物，VOCs 分析可作为肺癌诊断的补充检测手段。
